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Metallothionein immunostaining in adenoid cystic carcinomas of the salivary glands
Authors:Alves Sérgio Melo  Cardoso Sérgio Vitorino  de Fátima Bernardes Vanessa  Machado Vinícius Carvalho  Mesquita Ricardo Alves  Vieira do Carmo Maria Auxiliadora  Ferreira Aguiar Maria Cássia
Affiliation:1. Oral Pathology, School of Dentistry, Federal University of Minas Gerais (UFMG), Av. Antônio Carlos, 6627, Pampulha, CEP: 31270-901, Belo Horizonte/MG, Brazil;2. Oral Diagnosis Division, Department of Dentistry, State University of Montes Claros (UNIMONTES), Campus Universitário Prof. Darcy Ribeiro, Bloco 6, Vila Mauricéia, CEP: 39401-089, Montes Claros/MG, Brazil;1. Department of Medical and Surgical Science, Section of Dentistry, University of Catania, Catania, Italy;2. Department of Medical and Surgical Science, Section of Plastic Surgery, University of Catania, Catania, Italy;3. Department of Maxillofacial Surgery, University of Messina, Messina, Italy;4. Unit of Oral Pathology, School of Dentistry, University of Birmingham, Birmingham, United Kingdom;5. Department of Oral and Maxillofacial Sciences, School of Dentistry, “Sapienza” University of Rome, Rome, Italy;6. Universidade Federal Da Bahia Faculdade De Odontologia Laboratório De Patologia Cirúrgica, Bahia, Brazil;7. Division of Anatomy, Faculty of Dental Medicine, “Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania;8. Dipartimento di Neuroscienze, Scienze Riproduttive ed Odontostomatologiche, Institute of Oral Pathology, University of Napoli ‘Federico II’, Naples, Italy;9. Direttore Struttura Complessa di Anatomia Patologica, Dipartimento di Medicina Clinica e Sperimentale, Università Degli Studi di Foggia, Foggia, Italy;10. Department of Clinical and Experimental Medicine, Institute of Pathology and Cytopathology, University of Foggia, Foggia, Italy;1. Dentistry Department, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil;2. Dentistry Department, State University of Paraíba, Campina Grande, Paraíba, Brazil;3. Dentistry Department, Federal University of Campina Grande, Patos, Paraíba, Brazil;4. Oral Pathology Department, Federal University of Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil;1. Department of Oral and Maxillofacial Pathology, School of Dentistry, University of São Paulo, Avenida Professor Lineu Prestes, 2227, 05508-000, São Paulo, São Paulo, Brazil;2. Department of Pathology, São Leopoldo Mandic Institute and Research Centre, Rua José Rocha Junqueira, 13, 13045-755, Campinas, São Paulo, Brazil;1. Postgraduation Program in Public Health, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil;2. Postgraduation Program in Oral Pathology, Department of Dentistry, Federal University of Rio Grande do Norte, Natal, RN, Brazil;3. Postgraduation Program in Dentistry, Department of Dentistry, State University of Paraíba, Campina Grande, PB, Brazil;1. Oncology Division, Medicine Department, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil;2. Department of Pathology, Universidade Federal de São Paulo UNIFESP/EPM, SP, Brazil;3. Department of Biosciences, Universidade Federal de São Paulo UNIFESP, Santos, SP, Brazil;1. Department of Diagnosis and Surgery, Paulista State University (UNESP), Araraquara, São Paulo, Brazil;2. Oral Pathology, Department of Stomatology, Public Oral Health and Forensic Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil
Abstract:Metallothionein (MT) is a protein that has been studied in several tumors as a prognostic factor and as a potential myoepithelial cell marker in breast cancer. The aims of this study were to assess the immunohistochemical staining of MT in adenoid cystic carcinomas of the salivary glands (ACC), and to analyze possible morphological and quantitative variations among the solid, cribriform, and tubular histological subtypes. MT was investigated in 15 cases of ACC using the immunohistochemical technique. All of the cases expressed the MT antigen. This expression was noteworthy in cells depicting myoepithelial differentiation. ACC with predominant tubular pattern presented a significantly lower mean index of MT immunopositivity than did solid or cribriform subtypes, while these two latter groups did not differ in terms of MT expression. Our results suggest that MT may be an important tool for immunolocalization of myoepithelial tumor cells in salivary gland neoplasms.
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