失神经支配的腓肠肌组织中myostatin基因CDS部分序列克隆

目的 :克隆大鼠坐骨神经离断时失神经支配的腓肠肌组织myostatin基因CDS区部分片段。方法 :大鼠行坐骨神经离断术 ,术后 2周提取失神经支配的腓肠肌组织总RNA ,扩增myostatin基因CDS片段 ,克隆入T载体 ,并经序列测定。结果 :采用RT -PCR法 ,扩增出一特异产物与预期长度相符 ,T载体克隆测序与myostatin基因 1 0 0 %同源。结论 :采用RT -PCR和T载体技术可获得大鼠失神经支配的腓肠肌组织myostatin基因CDS部分片段的克隆

The Partial CDs Gene Clone of Myostatin From Denervated Gastrocn Emius of Rat

Objective:To obtain the gene clone of partial myostatin gene from denervated Gastrocnemius muscle of the rat. Methods:The partial CDS of myostatin was amplified by RT-PCR from denervated Gastrocnemius muscle of rats, and then was ligated into pGEM-T vector. The recombined fragment was sequenced.Results:The product of RT-PCR was 311bp which was expected. The recombined fragment was completely homogeneous with the myostatin gene reported.Conclusions:The partial CDS of myostatin gene had been cloned from the denervated Gastrocnemius muscle of rats using RT-PCR and T vector techniques.