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金黄葡萄球菌中毒休克综合征毒素1的研究
引用本文:王敏,李先平,付炅,范婧,唐爱国. 金黄葡萄球菌中毒休克综合征毒素1的研究[J]. 中华微生物学和免疫学杂志, 2008, 28(4)
作者姓名:王敏  李先平  付炅  范婧  唐爱国
作者单位:1. 中南大学湘雅二医院检验科,长沙,410011
2. 中南大学湘雅医学院医学检验系
摘    要:目的 应用PCR技术,检测金黄葡萄球菌的mecA基因和染色体上编码中毒休克综合征毒素1(TSST-1)的tst基因,了解tst基因的携带情况.方法 用PCR法对我院2006年8月-2007年5月临床分离的84株金黄葡萄球菌mecA基因和tst基因进行体外扩增,建立快速、特异、灵敏的检测产TSST-1耐甲氧西林金黄葡萄球菌(MRSA)的方法.结果 成功的对金黄葡萄球菌mecA基因和tst基因进行了检测,并进行基因测序.在我院84株受检金黄葡萄球菌中,41株金黄葡萄球菌的mecA基因扩增呈阳性,阳性株占48.81%.16株tst基因阳性,阳性株占19.05%.10株金黄葡萄球菌同时扩增出mecA基因和tst基因,阳性株占24.39%(10/41).结论 tst基因阳性株在临床分离的耐甲氧西林金黄葡萄球菌中占有较高的比例,应予以足够重视.

关 键 词:金黄葡萄球菌  中毒体克综合征毒素1  mecA基因  tst基因

Investigation on toxic shock syndrome toxin 1 from Staphylococcus aureus
WANG Min,LI Xian-ping,FU Jiong,FAN Jing,TANG Ai-guo. Investigation on toxic shock syndrome toxin 1 from Staphylococcus aureus[J]. Chinese Journal of Microbiology and Immunology, 2008, 28(4)
Authors:WANG Min  LI Xian-ping  FU Jiong  FAN Jing  TANG Ai-guo
Abstract:Objective To detect the mecA gene and tst gene of toxic shock syndrome toxin 1 (TSST-1)of Staphylococcus aureus by using PCR and to learn the carrier condition of tst gene.Methods The mecA gene and tst gene of Staphylococcus aureus strains that isolated from clinical sources in our hospital during August 2006 to May 2007 were amplified in vitro using PCR,and to establish the rapid,specific,and sensitive method of detecting tst gene of methicillin resistant Staphylococcus aureus(MRSA).Results The mecA gene and tst gene were detected,and were made the gene sequencing successfully.Forty-one of 84 strains had mecA gene(48.81%),16 of 84 strains had tst gene(19.05%),10 of 84 strains had both of them,and the positive rate was 24.39%(10/41).Conclusion The proportion of tst gene positive strains of MRSA iS high in clinic,and it must be paid more attention.
Keywords:Staphylococcus aureus  Toxic shock syndrome toxin 1  mecA gene  tst gene
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