乙型肝炎病毒基因分型与临床及TH1/TH2细胞因子相关性

目的 研究乙肝病毒感染者TH1/TH2细胞因子水平,比较慢乙肝患者HBV基因型与临床特征、TH1/TH2细胞因子免疫应答的相关性.方法 通过流式细胞技术检测102例乙肝病毒感染者及48例健康体检者IFN-γ及IL-4水平,并随机抽取50例慢乙肝患者采用实时荧光PCR法进行HBV基因分型.结果 乙肝病毒感染者IL-4水平较健康体检者明显升高（P＜0.001）;不同HBVDNA复制水平及HBeAg状态与TH1/TH2细胞因子无明显相关性（P＞0.05）;50例慢性乙型肝炎患者中基因B型34例（68%）,基因C型16例（32%）;HBV基因C型患者与基因B型患者相比,IL-4水平明显升高（P=0.018）,Th1/Th2比值降低,但差异无统计学意义（P=0.2262）.结论 TH1/TH2细胞因子水平差异对于解释HBV基因B型患者与基因C型患者临床转归差异可能提供了细胞免疫学依据.

The association between HBV genotyping and clinical characteristics and expression of TH1/TH2 cytokines

Objective To explore the association between HBV genotyping and clinical characteristics and expression of TH1/TH2 cytokines. Methods The expression of IL-4 and IFN-γ was detected with flow cytometry for 102 HBV infections and 48 healthy controls. 50 CHB patients were randomly selected for HBV genotyping with real-time fluorescence PCR assay. Results Higher expression of IL-4 in peripheral blood was detected in patients with HBV infection than healthy controls ( P ＜ 0. 001 ); No significant differences on expression of Th1/Th2 cytokines were observed in CHB patients with different HBV DNA levels or HBeAg status(P ＞ 0.05). There were 34 (68%)patients with genotype B infection and 16 (32%) with genotype C infection. Compared to patients with genotype B infection,the patients with genotype C infection showed higher levels of IL-4( P = 0. 018 ), and Th1/Th2 ratio decreased, but the difference was not statistically significant( P = 0. 2262). Conclusion The different expression of TH 1/TH2 cytokines may elucidate cellular immune response and clinical outcome difference between patients with genotype B infection and genotype C infection.