CMV-pp65抗原检测在乙肝相关性肝癌患者中的临床意义

目的:调查乙肝相关性肝癌患者CMV-pp65抗原血症情况,探讨CMV-pp65抗原检测的临床意义。方法:采用免疫组化二步法和酶联免疫吸附反应法(ELISA)检测30例乙肝相关性肝癌患者CMV-pp65抗原和CMV特异性IgM抗体;同时检测18例健康体检者的CMV-pp65抗原设为对照组,采用卡方检验进行统计分析。结果:30例乙肝相关性肝癌患者中CMV-pp65抗原血症阳性13例,阳性率43.3%(13/30);CMV特异性IgM抗体阳性2例,阳性率6.7%(2/30),经卡方检验,χ2=10.756,P<0.01,对比组之间的差异具有统计学意义。对照组18例健康体检者CMV-pp65抗原血症阳性0例,经卡方检验,χ2=10.697,P<0.01,对比组之间的差异具有统计学意义。结论:乙肝相关性肝癌患者CMV-pp65抗原血症阳性率高,提示活动性CMV感染;CMV-IgM抗体检测不适合肝癌患者活动性CMV感染的早期诊断。

The clinical significance of CMV-pp65 antigen detcetion in hepatitis B virus(HBV) related hepatocellular carcinoma patients

Objective: To investigate CMV-pp65 antigenemia in hepatitis B virus(HBV) related hepatocellular carcinoma patients and discuss the clinical significance of CMV-pp65 antigen detcetion.Methods: Immunohistochemistry two-step method and enzyme-linked immunosorbent assay(ELISA) were used to detect the CMV-pp65 antigen and CMV-specific IgM antibodies in 30 cases of HBV related hepatocellular carcinoma patients;Simultaneously the CMV-pp65 antigen of 18 healthy volunteers were detected as a control group.Chi-square test was used for statistical analysis.Results: The positive rate of CMV-pp65 antigen in HBV related hepatocellular carcinoma patients was 43.3%(13/30);while the positive rate of CMV-specific IgM antibodies was 6.7%(2/30).The difference had significance in statistics(χ2=10.756,P<0.01).In Healthy control group,0 in 18 cases was CMV-pp65 antigen positive.The difference was of significance in statistics(χ2=10.697,P< 0.01).Conclusion: Hepatitis B virus related hepatocellular carcinoma patients have high positive rate of CMV-pp65 antigenemia,suggesting active CMV infection;CMV-IgM antibody is not suitable for the early diagnosis of active CMV infection in patients with hepatocellular carcinoma.