二烯丙基二硫诱导人胃癌MGC803细胞凋亡及细胞周期阻滞的研究

目的　研究二烯丙基二硫 (diallyldisulfide ,DADS)诱导人胃癌MGC80 3细胞凋亡和对细胞周期的影响。方法　采用MTT法、丫啶橙染色、流式细胞仪等方法检测DADS对MGC80 3的增殖抑制 ,诱导凋亡以及细胞周期分布的影响。结果　MTT法显示 ,DADS对MGC80 3细胞有明显的抑制增殖作用 ,2 0、3 0、40mg·L- 1DADS作用MGC80 3细胞72h后 ,生长抑制率分别达 2 5 7%、58 6%、69% ;经 3 0mg·L- 1DADS作用MGC80 3细胞 48h后 ,用丫啶橙染色法观察到不同阶段的凋亡细胞形态学改变 ;流式细胞仪分析结果显示 ,DADS对MGC80 3细胞有明显的G2 /M期阻滞作用 ,3 0mg·L- 1DADS作用MGC80 3细胞 2 4h ,与对照组相比 ,可使G2 /M期细胞增加到 4倍多 ,且DADS呈时间依赖性诱导MGC80 3细胞凋亡 ,3 0mg·L- 1DADS作用MGC80 3细胞48h ,凋亡率从对照组的 3 5%升高到 3 9 5%。结论　DADS引起MGC80 3细胞G2 /M期阻滞并诱导凋亡可能是其抗肿瘤的机制之一

Effect of diallyl disulfide on apotosis and cell cycle arrest of human gastric cancer MGC-803 cells

AIMTo investigate whether DADS induce MGC803 cell apop tosis and cell cycle arrest. METHODSMGC803 cell growth inhibitio n was measured by MTT assay. Flow cytometry and acridine orange fluorescent stai ning method were used to determine the induction of apoptosis and the change of cell cycle. RESULTSMTT assay showed that adding 20,30,40 mg·L -1 DADS for 72 h suppressed MGC803 growth by 25 7%,58 6%,69 0% respective ly. Partial cells presented the characteristic morphological changes of apoptosi s under the fluorescent microscope. The apoptosis rate ncreased in time-depende nt manner. Flow cytometry analysis revealed that treating MGC803 cell with DADS significantly increased in the percentage of cells in the G 2/M phase. The proportion of cells in the G 2/M phase after treatment with 30 mg·L -1 DADS for 24 hours was comparable (46 0%), and more than four times that occu rring in untreated cells (9 9%). Furthermore, flow cytometry analysis also demonstrated that DADS induced apoptosis of MGC803 cell in time-dependent manner. T he pencentage of apoptotic cell was 3 53% after 0 h of 30 mg·L -1 DADS tr eatment. This pencentage of apoptotic cell rose steadily over time reaching 9 8 % after 24 h and 39 5% after 48 h. CONCLUSIONDADS could induce apoptosis of MGC803 cells and block the cell cycle at G 2/M phase.