三氧化二砷对人体胃肠癌细胞凋亡及P53、Bcl-2表达的影响

目的研究三氧化二砷（As2O3）在人体内诱导胃癌、结肠癌细胞凋亡及对凋亡相关基因P53、Bel-2表达的影响，探讨其抗肿瘤作用机制。方法采用原位末端转移酶标记技术和免疫组化法分别检测As2O3用药前后胃癌和结肠癌细胞凋亡指数和凋亡相关基因P53、Bcl-2的表达变化情况。结果As2O3用药后胃癌、结肠癌细胞凋亡率分别为15．53％和16．76％，与用药前比较均有显著性差异（P〈0．05）；用药后胃癌、结肠癌细胞P53蛋白阳性表达率分别为35．42％和30．83％，与用药前的38．08％和31．58％比较无显著性差异（P〉0．05）；用药后胃癌、结肠癌细胞Bcl-2蛋白阳性表达率分别为23．80％和16．90％，与用药前比较显著下调，二者均具有显著性（P〈0．05）。结论As2O3在人体内可诱导胃癌和结肠癌细胞凋亡发挥抗肿瘤作用，其机制可能是通过调节bcl-2基因的表达来实现。

The apoptosis and expression of P53 and Bcl-2 in human gastric and colorectal adenocarcinoma cells induced by arsenic trioxideon

Objective To study the impact of arsenic trioxide on the apoptosis and the changes of P53, Bcl-2 expression on of human gastric and colorectal adenocarcinoma cells induced by arsenic trioxide, and its mechanisms on of anti-tumor effect. Methods The expressions of P53, Bcl-2 and apoptosis induced by arsenic trioxide were examined respectively by immunohistochemistry method and TUNEL. Resuits Apoptosis percentage of post-chemotherapeutic gastric and colorectal adenocarcinoma ceils were was 15.53 % and 16.76%. Compared with pre-chemotherapy （ 6. 88%, 7. 11% ）, there is more significant than significant difference（ P 〈 0.05 ）. The differences were significant statisticallly. Expression of P53 was not changed by As203 （P 〉 0.05）. Compared with pre-chemotherapy（37. 16% in gastric carcinoma and 28.69% in eolorectal adenocarcinoma cells） ,the expression of post-chemotherapeutic Bcl-2 （23.80% in gastric carcinoma and 16.90% in colorectal adenocarcinoma cells） was down-regulated than. The differences were significant statistically （P 〈 0.05 ）. Coudusion As2 03 can induce apoptosis in of gastric and colorectal cancer cells,possibly, and its mechanisms may be through accommodating the expression of Bcl- 2 gene.