Dynamic observation of the effects of anti-keratin autoanti—bodies on the embedded autoepidermis

Autografts of skin of 3 rabbits were embedded in the subcutis of their backs.Samplingswere separately taken 2,4,6,8,10 and 12 d after grafting,A dynamic observation of the effectsof the anti-keratin autoantibodies(AK auto Ab)on the autoepidermis was made by HE,directimmunofluorescence(DIF)and peroxidase-antiperoxidase(PAP)stainings on frozen sections.It wasshown that the embedded autoepidermis could rapidly proliferate,extend and surround the keratinto form cysts.The specific IgG deposition was seen near the stratum corneum before the epidermiswas proliferated,and also there were obvious increases of IgG deposited in the granular layer ofproliferated epidermis,keratin and exfoliative keratinocytoplasm.But,after the keratin wascompletely blocked,it was found that the IgG decreased in the above areas.It is suggested that theconjugation of AK auto Ab with keratin may provide the earliest“biological information”of theabove-described reaction of epidermis.Dyskeratotic cells and hyaline corpuscles may be the resultsof interaction of the AK auto Ab of the exfoliative keratinocytes.