| 丙型肝炎病毒体外感染MT-2细胞的研究 |
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| 引用本文： | 陈智,刘勇,陈明华. 丙型肝炎病毒体外感染MT-2细胞的研究[J]. 中华肝脏病杂志, 1999, 7(3): 144-145 |
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| 作者姓名： | 陈智 刘勇 陈明华 |
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| 作者单位： | 浙江大学医学院附属第一医院传染病研究所!３１０００３(陈智，刘勇，陈明华，沃健尔，刘克洲，何南祥)，德国吕贝克医科大学微生物研究所(ＤＥＮＮＩＮＲｅｉｎｈａｒｄＨ，ＲＥＩＮＨＡＲＤＵｒｓａ) |
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| 摘 要： | 目的建立体外丙型肝炎病毒感染的ＭＴ－２细胞模型。方法用ＨＣＶＲＮＡ阳性的丙型肝炎患者血清感染人淋巴细胞株ＭＴ－２以ＨＣＶＲＮＡ阴性血清作对照，用ＲＴ－ＰＣＲ分析ＨＣＶ在体外细胞培养中的复制状态。结果用ＨＣＶ阳性血清感染的ＭＴ－２细胞在培养第７天开始检出ＨＣＶＲＮＡ，并一直持续阳性至第２８天；培养至第１０天可测出ＨＣＶ复制中间体，于第１８天消失。用ＨＣＶ阴性血清感染的ＭＴ－２细胞未检出ＨＣＶＲＮＡ。扩增产物经Ｓｏｕｔｈｅｒｎ转移后用ＨＣＶ探针杂交证实ＨＣＶ特异性序列。结论ＨＣＶ可在ＭＴ—２中短期复制。
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| 关 键 词： | 丙型肝炎病毒 体外 感染 MT－2 |
In vitro study on MT2 cell infected by hepatitis C virus |
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| CHEN Zhi, LIU Yong, CHEN Minhus et al.. In vitro study on MT2 cell infected by hepatitis C virus[J]. Chinese journal of hepatology, 1999, 7(3): 144-145 |
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| Authors: | CHEN Zhi LIU Yong CHEN Minhus et al. |
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| Affiliation: | Institute of Infectious Diseases, First Affiliated Hospital, Medical Collage Zhejiang University, Hangzhou. |
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| Abstract: | Objective To establish an MT-2 cell model infected by HCV in vitro. Methods HumanT-lymphocyte cell line MT-2 was infected with HCV--RNA positive serum and detected for HCV RNAby RT-PCR 1, 4, 7, 10, 14, 18, 21, 24, 28, 31, 35 and 39 days after the infection. PCR products weredetermined by southern blot. MT-2 cell infected with HCV--RNA negative serum was used as control.Results HCV-RNA begun to turn positive at 7 days postinoculate (p.i.) till at least 28 days(p.i.). Intracellular negative--stranded HCV--RNA was detected from 10 to 18 days (p.i.). No HCV RNA was detectedin the control group. Conclusion HCV may replicate and express in MT--2 cell for a short period. |
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| Keywords: | Hepatitis C virus in vitro infection MT-2 |
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