兔自体肌腱细胞与碳纤维联合培养后体内植入实验研究

为了观察兔自体肌腱细胞与碳纤维联合培养后植入体内的改变，采用自体肌腱细胞传代后与碳纤维编织带联合体外培养，植入兔体内，分期观察了肌腱细胞的形态学改变及合成胶原的类型。结果发现：体内植入后肌腱细胞能继续增殖，并能合成Ⅰ型胶原。同时发现肌腱细胞在植入４周后脱离碳纤维支架，在编织带间增殖、合成胶原，其胶原纤维相互衔接，形成了致密的组织结构。桥接肌腱处，胶原纤维互相衔接，表明植入体与受体肌腱已愈合。扫描电镜下观察肌腱细胞在碳纤维间排列整齐、均匀，胶原纤维间相互连接形成网状，纤维条索主体与碳纤维方向一致。透射电镜下细胞核核仁清晰，细胞器丰富。为进一步研究有生命的人工材料提供了依据。

EXPERIMENTAL STUDY IN VIVO ON IMPLANTATION OF AUTOGENOUS TENDON CELLS AFTER COMBINING CULTURE WITH CARBON FIBERS /

In order to investigate the possibility of repairing injuried tendon with living artificial tendon, after combining culture, subcultured autogenous tendon cells with carbon fibers were implanted into the calcaneous tendon of rabbits. In different stages, the synthesis of type I collagen and their relevant morphological changes were observed. The results showed as follows: after implantation, tendon cells continued proliferating. Four weeks after implantation, tendon cells were detached from the carbon fibers and proliferated and produced collagen among the carbon fibers. The collagen fibrils were linked with each other to formed a dense structure. In the linkage site, the collagen fibrils originated from the implants joined to that from the ruptured end of the tendon, which meaned that the implant was healed with the recipient tendon. Observed under scanning electronic microscope, the tendon cells were lined among the carbon fibers evenly and in order, the collagen fibrils joined each other and formed an network, the fibrils were lined parallel to the carbon fibers. Under transparent electron microscope, the nucleolus were clear and organelle were abundant.