Evaluation of Cassia occidentalis for in vitro cytotoxicity against human cancer cell lines and antibacterial activity |
| |
Authors: | Bhagat Madhulika Saxena Ajit Kumar |
| |
Affiliation: | School of Biotechnology, University of Jammu, Jammu, India. |
| |
Abstract: | Objective:To evaluate the in vitro cytotoxicity and antibacterial properties of Cassia occidentalis (whole plant) via alcoholic, hydro-alcoholic, and aqueous extracts against eight human cancer cell lines from six different tissues and four bacterial strains.Material and Methods:in vitro cytotoxicity against the human cancer cells, cultured for 48h in presence of different concentrations C. occidentalis extracts and percentage of cell viability, was evaluated using the sulforhodamine-B (SRB) assay. The antibacterial activity was performed using the standard protocol against bacterial strains.Results:It was observed that aqueous extract of C. occidentalis (whole plant) had more potential than hydro-alcoholic and alcoholic extracts against HCT-15, SW-620, PC-3, MCF-7, SiHa, and OVCAR-5 human cancer cell lines at 100, 30, and 10 μg/ml in a dose-dependent manner. The hydro-alcoholic extract showed potential against Bacillus subtillis.Conclusion:The plant can be explored for the possible development of lead molecules for drug discovery. |
| |
Keywords: | Antibacterial activity Cassia occidentalis cytotoxicity |
本文献已被 PubMed 等数据库收录! |
|