无电流斑电击死兔的红细胞与血管内皮的扫描电镜观察

目的 探讨无电流斑电击死动物的红细胞与血管内皮细胞的形态学变化.方法 建立两种无明显电流斑电击死动物模型.模型A:用220 V或110 V交流电直接电击浸湿的右前肢与左后肢致死.模型B:用220 V或110 V交流电电击水中兔致死.取电击死兔血液及肺动脉与主动脉,扫描电镜观察红细胞及内皮细胞形态学变化.结果 两种电击死动物模型兔的红细胞与血管内皮细胞均可见穿孔,红细胞穿孔细胞指数最高,其次为肺动脉内皮细胞,主动脉穿孔细胞穿孔指数较肺动脉内皮与红细胞稍低,而对照组则未见穿孔现象.结论 细胞穿孔可以作为无明显电流斑电击死的一个诊断指标.

Scanning Electron Microscopic Observation of Erythrocytes and Endothelial Cells of Electrified Death Rabbits

Objective To identify electrified death of animals without electric marks.Methods Two animal models(model A and model B) of electrified death without electric marks were established.Model A: right anterior limb and left hind limb of rabbits were soddened by 0.1 mol/L PBS,and two pieces of wet gause which were soaked in the 0.1 mol/L PBS were winded on the right anterior limb and left hind limb of them,respectively,and then two nake wires were winded outside of the gause;then,the two wires were connected to the alternating current of 220 voltage(A1 group) or 110 voltage(A2 group) electrified.Model B: two nake wires were fixed at the bilaterals of an oblong container,then a small quantities water were infunded into it,about 1.5 cm deep,and then,one rabbit was put in each time;finally,the two wires were connected to the alternating current of 220 voltage(B1 group) or 110 voltage(B2 group) electrified.The erythrocytes and endothelium of aorta and pulmonary artery of the animals were observed under scanning electron microscopes.Results Pores were found on the surface of the erythrocytes and endothelial cells of aorta and pulmonary artery of the electrified animals,which was absent in the control animals.The erythrocytes had the greatest perforated cell index among the three cells,followed by the pulmonary endothelium.Conclusion The perforation phenomena can serve as an evidence of electrified death when no electric mark is observable.