内淋巴囊对外源性TD抗原的清除作用

目的:探讨内淋巴囊对外源性胸腺依赖性(TD)抗原的吞噬和清除作用。方法:用S-D大鼠30只,以TD抗原钥孔(?)血蓝蛋白(KLH)全身免疫后2周,再次在内耳接种相同抗原,分别于此后1 h、3 h、1 d、3 d、7 d和14 d,取颞骨作组织学处理、冰冻切片,用抗KLH单克隆抗体,运用免疫组织化学技术,观察内耳免疫应答过程中内淋巴囊腔内KLH的动态变化。结果:内耳抗原接种后3 h,内淋巴囊出现KLH;第1～7 d在内淋巴囊的抗原被捕捉吞噬和递呈;第14天内淋巴囊仅发现微弱的抗原染色。结论:在内耳的二次应答中,内淋巴囊能够吞噬、处理和清除外源性抗原说明其具有免疫防御和免疫应答功能。

Clearance function of endolymphatic sac to extraneous TD-antigen

Objective:To study the clearance function of the rat endolymphatic sac to TD antigen. Method: 30 healthy adult S-D rats were employed in the experiment. At two weeks after being sensitized systemically with keyhole limpet hemocyanin (KLH) (Thymus-dependent antigen), the animals were inoculated into the labyrinth through cochlea base tune with the same antigen. At 1,3 h, 1, 3,7 and 14 day after labyrinth vaccination, the rats were sacrificed and the temporal bones were removed, then the frozen-sections of the decalcified samples were examined respectively. Using Anti-KLH McAb,the SABC immunohistochemical methods were utilized to investigate the changes of KLH in the endolymphatic sac. Result: At 3 h after labyrinth vaccination the antigen appeared in the endolymphatic sac. While the KLH were trapped and phagocytized in the endolymphatic sac at 1-7 day after labyrinth vaccination, the antigen in the cochleae gradually reduced. At the 14th day only feeble KLH positive stains can be found in the endolymphatic sac. Conclusion:The rat endolymphatic sac can phagocytize,dispose and removal extraneous antigen, which verifies the immune defense function of endolymphatic sac in inner ear immune response.