| 树舌多糖GF对HepA瘤细胞N-ras基因表达的影响 |
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| 引用本文： | 方志伟,于英君. 树舌多糖GF对HepA瘤细胞N-ras基因表达的影响[J]. 中医药学报, 2005, 33(3): 60-61,i001 |
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| 作者姓名： | 方志伟 于英君 |
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| 作者单位： | 1. 哈尔滨医科大学,黑龙江,哈尔滨,150080
2. 黑龙江中医药大学,黑龙江,哈尔滨,150040 |
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| 摘 要： | 目的:探明树舌多糖GF对HepA瘤组织N-ras基因表达的影响.方法:运用原位杂交法、SP免疫组化染色技术及多媒体彩色病理图文分析系统测定HepA瘤细包中N-ras mRNA量及N-Ras蛋白的表达量.结果:树舌多糖组、猪苓多糖组中N-ras mRNA量及Ras蛋白的表达量均显著低于荷瘤组(P＜0.01),树舌多糖组与猪苓多糖组无显著性差异.结论:初步认为树舌多糖GF可通过降低N-rasmRNA量及Ras蛋白的表达,而抑制HepA瘤细胞的增殖.
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| 关 键 词： | 树舌多糖GF HepA肿瘤细胞 N-ras基因 |
| 文章编号： | 1002-2392(2005)03-0060-01 |
Effect of Ganoderma appanatum polysaccharides GF on Ras Protein experession in mouse HepA tumour |
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| FANG Zhi-Wei,YU Ying-jun. Effect of Ganoderma appanatum polysaccharides GF on Ras Protein experession in mouse HepA tumour[J]. Acta Chinese Medicine and Pharmacology, 2005, 33(3): 60-61,i001 |
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| Authors: | FANG Zhi-Wei YU Ying-jun |
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| Abstract: | Objective:To study the effect of GAPSGF on N-ras mRNA and N-ras protein expression in HepA tumour.Methods:Immunohistochemical staining and ISH were used to determine the effect of Ganoderma appanatum polysaccharides GF on N-ras mRNA and N-ras protein expression in mouse HepA tumor.Results:The average levels of N-ras mRNA and N-ras protein expression in GAPSGF group and PUPS group have much difference with control group.That is to say,GAPSGFC and PUPS can significantly reduce the expression of N-ras mRNA and N-ras protein expression in HepA cells.onclusion:Regulate the N-ras oncogene expression is one of the mechanisms of antitumor activity. |
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| Keywords: | Ganodermaw appanatum polysaccharides GF(GAPSGF) liver tissue HepA tumor N-ras oncogene |
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