Mucosal metabolism in ulcerative colitis and crohn's disease |
| |
Authors: | Maeve M Duffy PhD Mark C Regan MD P Ravichandran MB BCh Conor O'Keane MD Michael G Harrington PhD John M Fitzpatrick MCh Dr P Ronan O'Connell MD |
| |
Institution: | (1) From the Department of Surgery, Mater Misericordiae Hospital and University College, Dublin, Ireland;(2) Department of Biochemistry, Mater Misericordiae Hospital and University College, Dublin, Ireland;(3) Department of Pathology, Mater Misericordiae Hospital and University College, Dublin, Ireland |
| |
Abstract: | PURPOSE: Colonic mucosal metabolism of butyrate may be impaired in ulcerative colitis. In this study we sought to confirm this observation, to determine if a similar change occurs in Crohn's colitis, and to establish whether a panenteric disorder of butyrate metabolism exists in either condition. METHODS: With use of a microculture technique, mucosal metabolic fluxes of14C]-labeled butyrate and14C]-labeled glutamine were measured as14C] carbon dioxide production in mucosal biopsy specimens from the colon and ileum in patients with ulcerative colitis, Crohn's colitis, and healthy bowel. Results were expressed as pmol/µg biopsy DNA/hour. RESULTS: In the colon the mucosal metabolic fluxes of both butyrate and glutamine are reduced in both ulcerative colitis and Crohn's colitis compared with healthy controls. These changes were most marked in the presence of moderate to severe mucosal inflammation, there being no significant difference in mucosal metabolic flux between mildly inflamed mucosa and healthy controls. In the ileum the mucosal metabolic fluxes of butyrate and glutamine did not differ between healthy controls and those with either ulcerative colitis or Crohn's colitis. CONCLUSIONS: Changes in colonic mucosal metabolism of butyrate and glutamine in inflammatory bowel disease occur as a consequence of the inflammatory process and are not peculiar to ulcerative colitis. Ileal mucosal metabolism is unchanged in ulcerative colitis and Crohn's colitis, indicating the absence of a panenteric abnormality of mucosal metabolism in these two conditions.Supported by the Mater College, Dublin, Ireland.Portions of this work were read at the American Gastroenterological Association San Francisco, California, May 19 to 24, 1996, and an abstract was published in Gastroenterology 1996;110:A900. |
| |
Keywords: | Butyrate Glutamine Metabolism Ulcerative colitis Crohn's disease |
本文献已被 SpringerLink 等数据库收录! |
|