褪黑素对海洛因依赖大鼠淋巴细胞增殖和IL-2产生的影响

目的　观察褪黑素 (MT)对海洛因 (Her)依赖大鼠淋巴细胞增殖和IL 2产生的影响。方法　以剂量递增连续scHer建立海洛因大鼠依赖模型 ,并同时随机设一组给予MT保护。 42d后随机分组 ,设MT治疗组、美沙酮治疗组、自然戒断组、依赖组 ;另选正常大鼠为溶媒对照组。采用MTT法观察MT对ConA诱导的大鼠脾淋巴细胞增殖反应和IL 2的产生。结果　MT保护组 37 5mg·kg-1,bid与依赖组比较 ,对ConA诱导的T淋巴细胞增殖反应和IL 2的产生有明显的促进作用 ;MT 12 5、37 5、6 2 5mg·kg-1,bid 3个剂量组与美沙酮治疗组、自然戒断组比较 ,对ConA诱导的T淋巴细胞增殖反应和IL 2的产生均有不同程度的促进作用。结论　MT对海洛因造成的细胞免疫功能低下可能有预防和逆转的作用。

Effects of melatonin on lymphocyte proliferation and IL-2 production in heroin-dependent rats

AIM To observe the effects of melatonin(MT) on lymphocyte proliferation and IL 2 production in heroin dependent rats. METHODS The heroin dependent rats model was established by SC administered heroin in gradually applied load and other group of heroin dependent rats was taken MT as protected group. After 42 days, the heroin dependent rats were random divided into MT treated group, Methadone treated group, natural detoxification group, heroin dependent group and then saline group was served as controls. MTT colorimetric assay was employed to determine the lymphocyte proliferation and IL 2 production in vitro induced by concanavalin A (Con A). RESULTS The protected group by using MT (37 5 mg·kg -1 ,bid), compared with heroin dependent group, could enhance the proliferation and IL 2 production in rats spleen lymphocytes. MT treated group (12 5、37 5、62 5 mg·kg -1 ,bid), compared with methadone treated group and natural detoxification group, could enhance the proliferation IL 2 production in rats spleen lymphocytes in various degrees.CONCLUSION MT may have preventive and reverse effects on heroin induced indsufficiency of immunocyte functions.