Prohibitin 3'-UTR 基因多态性与粤东乳腺癌发病风险的研究

目的： 探讨Prohibitin (PHB) 3'-非翻译区 (untranslated region，UTR)基因多态性与粤东汉族女性乳腺癌易感性的关系。方法：采用病例-对照研究，收集231例乳腺癌患者和169例健康对照，提取血液基因组DNA，利用高分辨率熔解曲线 (high resolution melting，HRM)法对PHB基因单核苷酸多态性位点rs1049620、rs9893420、rs4987083进行基因分型，并对检测结果进行非条件Logistic回归分析和χ2检验。结果：PHB基因rs9893420和rs4987083位点在病例组和对照组人群均表现为单一基因型，rs1049620三种基因型G/G、A/G、A/A在病例组和对照组间分布差异均无统计学意义(P>0.05)，在不同组织病理学分型和不同激素受体表达病例中分布差异亦无统计学意义(P>0.05)。结论：未发现PHB基因3'-UTR SNP与粤东汉族女性乳腺癌易感性存在关联。

Prohibitin 3'-untranslated region polymorphism and breast cancer risk in easter n Guangdong of souther n China

OBJECTIVE: To determine the association of Prohibitin (PHB) 3'- untranslated region (UTR) polymorphism with the risk of breast cancer in eastern Guangdong of southern China．METHODS：A case-control study of 231 breast cancer patients and 169 healthy controls were enrolled into this study. Genomic DNA was extracted from whole blood cells. Single nucleotide polymorphisms of rs1049620，rs9893420 and rs4987083 were determined by the high resolution melting (HRM) method．Data were analyzed via Logistic regression analysis and Chi-square test. RESULTS：PHB gene at rs9893420 and rs4987083 sites showed single genotype in breast cancer group and control. There were no significant differences in frequencies of G/G，A/G and A/A at rs1049620 between patients and controls(P>0.05). Meanwhile，no significant correlation could be found between rs1049620 SNP and clinico-pathologic characteristics of breast cancer such as histological classification and expression of hormone receptor. CONCLUSION： Our results do not lend support to the hypothesis that PHB polymorphism contributes to susceptibility of breast cancer.