姜黄素对胆管癌 MZ-Cha-1细胞凋亡的诱导作用

目的： 研究姜黄素对胆管癌MZ-Cha-1细胞凋亡的诱导作用。方法：用0、10、20、40 μg/mL姜黄素处理MZ-Cha-1 细胞12、24、36 h后，应用噻唑蓝 (MTT)检测姜黄素对MZ-Cha-1细胞的抑制情况，再选择10 μg/mL姜黄素处理MZ-Cha-1细胞24 h后应用Giemsa和Hoechst33258染色检测MZ-Cha-1细胞的凋亡情况，应用流式细胞术检测细胞周期分布。结果：与对照组相比较，各姜黄素处理组对胆管癌MZ-Cha-1细胞有明显的抑制作用，并显示出对剂量和时间的依赖效应 (P<0.05)。10 μg/mL姜黄素诱导处理24 h后，经Giemsa染色显示细胞体积缩小、核固缩、染色质凝聚等显著的凋亡特征；细胞核经Hoechst33258 染色出现浓染致密的固缩形态和颗粒状荧光；细胞周期检测出现细胞被阻滞在G0/G1期，G2/M期细胞较对照组显著减少 (P<0.05)。结论：姜黄素能够有效诱导人胆管癌MZ-Cha-1细胞的凋亡。

Effect of curcumin on proliferation of human bile duct carcinoma MZ-Cha-1 cells

OBJECTIVE: To study the apoptotic effects of human bile duct carcinoma MZ-Cha-1 cells induced by curcumin. METHODS：After the MZ-Cha-1 cells induced by 0，10，20 and 40 μg/mL curcumin for 12，24，36 h，the apoptotic effects of curcumin were studied using the methods of MTT to detect the curcumin on MZ-Cha-1 cell suppression，and selected 10 μg/mL of curcumin treatment MZ-Cha-1 cells after 24 h，using Giemsa staining and Hoechst33258 dye to assess MZ-Cha-1 cell apoptosis，using flow cytometry to detect the cell cycle distribution. RESULTS：Compared with control group，the curcumin on MZ-Cha-1 bile duct carcinoma cell had obvious inhibitory effect，and in dose- and time- dependent mamer (P<0.05). Proliferation of MZ-Cha-1 cells could be inhibited by 10 μg/mL curcumin after 24 h. Light microscope showed that the morphology of the cells treated with curcumin appeared shrunken. Concentrated cell nucleus appeared granular and fluorescent by Hoechst33258 staining. Flow cytometry analysis showed that G0/G1 phase was increased and G2/M phase decreased (P<0.05). CONCLUSION：This study suggested that curcumin could induce apoptosis of the human MZ-Cha-1 cells effectively.