重症监护病房泌尿系感染产超广谱β-内酰胺酶大肠埃希菌基因分析

目的了解重症监护病房（ICU）泌尿系感染产超广谱β-内酰胺酶（ESBLs）大肠埃希菌的耐药性和基因型。方法收集2006年2月至8月从ICU中段尿培养中分离的20株产ESBLs的大肠埃希菌进行6种β-内酰胺酶基因的检测。结果20株菌中检出TEM基因11株（55％），CTX-M-1基因10株（50％），CTX-M-9基因3株（15％），SHV基因2株（10％），OXA-1基因1株（5％），OXA-10基因未检出。结论我院ICU泌尿系感染大肠埃希菌产ESBLs基因主要是TEM和CTX-M-1基因，其次为CTX-M-9和SHV基因，OXA-1基因阳性率低，而OXA-10基因未检出。

The gene analysis of Escherichia coli producing ESBLs isolated from urinary system of patients in intensivecare unit

Objective To investigate the drug resistance and the genotype of Escherichia coli （ E. coli） producing extended-spectrum β-lactamases（ESBLs） isolated from urinary system of patients in intensive care unit（ICU）. Methotis Six types of β-lactamase genes of 20 strains of E. coli producing ESBLs isolated from mid-stream urine culture were detected from February to August 2006. Results Among 20 strains of E. coli, the detection number of TEM, CTX-M-1, CTX-M-9, SHV, OXA-1 was 11, 10, 3, 2 and 1 respectively. Accordingly, the detection rate of TEM, CTX-M-1, CTX-M-9, SHV, OXA-1 was 55% , 50% , 15% , 10% and 5% respectively. The OXA-10 gene was not detected. Conclusions The genes of E. coli producing ESBLs isolated from urinary system in ICU of our hospital are mainly TEM and CTX-M-1, secondly CTX-M-9 and SHV. The positive rate of OXA-1 gene is low, while OXA-10 gene is not found.