应用荧光原位杂交技术对胚胎植入前行21—三体检查的研究

目的 避免移植染色体异常胚胎及提高体外受精-胚胎移植（IVF-ET）的质量。方法 应用DSCRCosmidDNA特异性探针，借助于荧乐原位杂交技术对20对35岁以上行IVF助孕夫妇的植入前胚胎进行21-三体检查。结果 在20例对夫妇中10对夫妇的胚胎成功地进行了植入前胚胎21-三体检查，其中8对夫妇的胚胎被证明为正常，给予常规移植，移植的8例胚胎中2例妊娠，其中1例流产，另1例正在妊娠中；2对夫妇的胚胎被证明为正常，给予常规移植，移植的8例胚胎中2例妊娠，其中1例流产，另1例正在妊姑吉，2对夫妇的胚胎被检查出21-三体，未给予移植。结论 在行IVF助孕的高龄妇女中，进行胚胎植入前21-三体检查是必要的。

Detection of trisomy 21 by fluorescent in-situ hybridization for preimplantation genetic diagnosis

Objective\ To avoid transferring embryo with chromosomal aberration and improve quality of IVF-ET.Methods Our research was performed by fluorescent in-situ hybridization(FISH) for preimplantation diagnosis of trisomy 21 in 20 couples who were over 35 years old. The special DSCR Cosmid DNA probe was applied. Results\ It was successful to analyse chromosomes from a single cell in 10 of 20 couples. There were normal embryos in 8 of 10 couples and their embryos were transferred. 2 of 8 couples had pregnancy, but one miscarriage occurred and the other was in a normal pregnancy. Trisomy 21 was detected in 2 of 10 couples and no embryo was transferred. Conclusion\ It is necessary to perform preimplantation genetic diagnosis for IVF patients of advanced maternal age.