阻塞性睡眠呼吸暂停犬模型肿瘤坏死因子-α与左心室功能的关系

目的:研究阻塞性睡眠呼吸暂停综合征(OSAS)犬模型血清中肿瘤坏死因子-α(TNF-α)含量的变化及其与心功能的关系。方法:设计OSAS犬模型,实验在6只正常杂种犬上进行。用ELISA法检测其血清中TNF-α含量。心脏彩超测量左室射血分数(LVEF)、左室舒张末期容积(LVEDV)。结果:TNF-α水平较实验前升高(P<0.05),LVEF、LVEDV、SV、HR、E/A值均较干预前降低(P<0.05)。TNFα-与LVEF呈负相关(r=-0.883,P<0.05),与LVEDV呈正相关(r=0.735,P<0.05)。结论:TNFα-可能在OSAS的心血管的发病中起着重要作用。

Relationship Between TNF-alpha and Left Ventricular Function in Dogs with Obstructive Sleep Apnea

Objective: To study the effects of tumor necrosis factor-alpha(TNF-α) on the cardiovascular function in dogs with obstructive sleep apnea syndromes(OSAS).Methods: Experiments were performed in six mature male Mongrel OSAS dogs.Serum concentrations of TNF-α of each dog were measured by ELISA immunoassay.The cardiovascular function indices as left ventricle ejection fraction(LVEF),left ventricle end-diastolic volume(LVEDV),stroke volume(SV),heart rate(HR) and E/A were measured by using echocardiography.Results: Serum concentrations of TNF-α increased significantly(P<0.05),and LVEF,LVEDV,SV,HR and E/A decreased compared with that of three months ago respectively(all P<0.05).TNF-α was negatively related with LVEF(r=-0.883,P<0.05) but positively with LVDEV(r=0.735,P<0.05).(Conclusion:) The increase of serum concentrations of TNF-α may play an important role in the pathogenesis of cardiovascular diseases in OSAS.