川芎地上部位的质量评价

目的:基于指纹图谱和含量测定对川芎地上部位(茎叶)进行质量评价.方法:采用Kromasil C18色谱柱(250mm×4.6mm,5μm),以0.1％磷酸水溶液(A)-乙腈(B)为流动相,梯度洗脱,流速:1.0mL/min,进样量20μL,柱温35℃,检测波长254nm,建立HPLC指纹图谱;采用Kromasil C1...

Quality evaluation of the aerial parts of Chuanxiong Rhizoma

Objective: The research on the quality evaluation of the aerial parts of Chuanxiong Rhizoma involves two aspects: fingerprint analysis and content determination. Methods: The fingerprint was performed on Kromasil C18(250 mm×4.6 mm, 5μm), the mobile phase was 0.1%phosphoric acid aqueous solution(A)-acetonitrile(B) at a rate of 1.0 mL/min, the sample size was 20μL, the column temperature was 35°C, and the detection wavelength was 254 nm. The content determination was performed on Kromasil C18(250 mm×4.6 mm, 5μm), the mobile phase was aqueous solution(A)-methanol(B)(34∶66) at a rate of 1.0 mL/min, the sample size was 10μL, the column temperature was 35°C, and the UV absorbance was monitored at 330 nm and 237 nm to determinate the content of ligustilide and n-butylidenephthalide. Results: There were 20 common peaks in the fingerprint of 13 batches of samples, with the similarities of more than 0.89. The content of ligustilide and n-butylidenephthalide of 13 batches of samples were 0.0642%~0.2633% and 0.0057%~0.0325%, respectively. Conclusion: The method is simple, accurate and reproducible, and can be used as a reference method for quality evaluation and quality control of the aerial parts of Chuanxiong Rhizoma.