白藜芦醇对梗阻性黄疸大鼠肝功能的保护作用

目的 研究白藜芦醇对梗阻性黄疸大鼠肝功能的保护作用及相关作用机制.方法 用胆总管结扎术的方法建立梗阻性黄疸大鼠模型,将成功构建的梗阻性黄疸大鼠随机分为模型组(n=16)与实验组(n=16),假手术组(n=16)仅开腹游离胆总管但不做结扎.实验组大鼠灌胃白藜芦醇(20 mg·kg-1·d-1),假手术组与模型组灌胃等量生...

Protective effect of resveratrol on liver function in rats with obstructive jaundice

Objective To investigate the protective effect of resveratrol on liver function in obstructive jaundice rats and its related mechanism.Methods The common bile duct ligation was used to establish the obstructive jaundice rat models,the successfully constructed obstructive jaundice rats were randomly divided into model group(n=16)and test group(n=16).Sham-operation group(n=16)only opened the free common bile duct without ligation.Rats in test group were administrated with resveratrol(20 mg·kg^-1·d^-1),sham-operation group and model group were administrated with the same amount of normal saline for 2 weeks.The levels of serum glutamic-pyruvic transaminase(GPT),glutamic-oxaloacetic transaminase(GOT),direct bilirubin(DBIL)and total bilirubin(TBIL)were measured by fully automatic biochemical analyzer;hematoxylin-eosin(HE)staining was used to observe the pathological changes of liver tissues in rats;the serum tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels were detected by enzyme-linked immunosorbent assay(ELISA);Western blotting(Wb)method was used to detect the expression levels of inducible nitric oxide synthase(i NOS)and nuclear transcription factor-κBp65(NF-кBp65)protein in rat liver tissues.Results The serum GPT levels in sham-operation group,model group and test group were(35.62±7.43),(241.25±19.22),(114.83±6.42)U·L^-1;GOT levels were(111.36±12.43),(562.73±48.64),and(312.43±19.65)U·L^-1;DBIL levels were(7.24±1.32),(263.51±17.22),(141.33±12.52)μmol·L^-1;TBIL levels were(9.16±1.43),(342.61±36.84),(181.22±19.86)μmol·L^-1;the relative expression levels of i NOS protein in liver tissues were 0.15±0.06,0.96±0.04,0.68±0.13;the relative expression levels of NF-кBp65 protein were 0.09±0.05,0.73±0.11 and 0.32±0.12,all with significant difference(all P<0.05).Conclusion Resveratrol can effectively improve the level of biochemical indicators of liver function in obstructive jaundice rats,inhibit the inflammatory response,thereby protect the liver and improve liver function,and the mechanism may be related to the reduction of i NOS and NF-кBp65 protein expression in liver tissues.