平刺与直刺三阴交穴对寒凝证类痛经大鼠子宫微循环及NO、NOS的影响

目的:观察平刺、直刺三阴交穴对寒凝证类痛经模型大鼠血清、子宫组织中一氧化氮(N0)及其合成酶(NOS)的影响,探讨不同刺法即刻镇痛作用的机制.方法:60只SD雌鼠随机分为空白组、模型组、直刺组、平刺组,每组15只.采用全身冷冻加雌二醇注射法制备寒凝证类痛经模型.针刺组分别直刺或平刺“三阴交”穴,留针20min.记录各组...

Effects of transverse and perpendicular needling at Sanyinjiao(SP6)on the uterine microcirculation and NO,NOS in cold congealing dysmenorrhea rats

Objective: To observe the effects of transverse needling and perpendicular needling at Sanyinjiao(SP6) on nitric oxide(NO) and its synthetase(nitric oxide synthase, NOS) in the serum and uterus tissue of cold congealing dysmenorrhea rats, and to explore its underlying mechanisms. Methods: A total of 60 SD female rats were randomly divided into blank group, model group, perpendicular needling group and transverse needling group, 15 rats in each group. The cold congealing dysmenorrhea rat model was prepared by systemic freezing and estradiol injection. Sanyinjiao(SP6) was needled perpendicularly or transversely in corresponding groups, and the needles were retained for 20 min. The writhing reaction and of rats in each group were recorded. The uterine microcirculation was observed by the ultra-high speed dynamic visualization microcirculation research system. Biochemical colorimetry was used to detect the levels of NO and NOS in serum of rats, eNOS expression in uterus tissues was detected by Western blot. Results: Compared with the blank group, the writhing latency was shortened and writhing score were significantly increased(P<0.01), the speed of blood flow in the microvessels slowed down(P<0.01), diameters of the uterine microvessels and capillaries(cap) shrank(P<0.01, P<0.05) in model group. Compared with the model group, the writhing latency were prolonged(P<0.05, P<0.01) and the writhing score were significantly reduced(P<0.01), and microvascular and cap diameters were significantly bigger(cap diameter at 5 minutes except)(P<0.01) in 2 acupuncture groups. Compared with perpendicular needling group, transverse needling improved the microflow state at 15 min after needling(P<0.01, P<0.05), and dilated the microvascular diameters at 35 min after needling(P<0.01). There were no statistically differences in the writhing latency and score between 2 acupuncture groups. Compared with the blank group, NO and NOS contents in the serum and eNOS expression in uterus tissues in the model group were significantly decreased in model group(P<0.01). After needling, NO and NOS contents in serum and eNOS expression in uterus tissues were increased in 2 acupuncture groups compared with the model group(P<0.01, P<0.05). NOS content and eNOS expression in transverse needling group were significantly higher than those in perpendicular needling group respectively(P<0.05, P<0.01). Conclusion: Both perpendicular needling and transverse needling at Sanyinjiao(SP6) show immediate analgesic effect on cold congealing dysmenorrhea rats. Transverse needling shows better effects on improving uterus microcirculation, and the underlying mechanism may be related to its effects of increasing NO and NOS content in the serum and upregulating eNOS expression in uterus.