钙对去势骨质疏松大鼠肠道菌群结构的影响

目的研究钙对去势骨质疏松大鼠肠道菌群结构的影响。方法30只SPF级雌性Wistar大鼠,按体重随机分为假手术组、模型组、碳酸钙补充组,去卵巢建立去势骨质疏松大鼠模型。造模1周后开始灌胃,碳酸钙补充组灌胃280mg/mL碳酸钙溶液10mL/kg,钙摄入量为1.34g/kg,其他两组灌胃等量无菌水,每日1次。连续灌胃12周后处死动物,取左侧股骨,用双能X射线骨密度仪测定骨密度;无菌提取粪便DNA,利用Illumina Miseq平台进行高通量测序。结果与假手术组相比,模型组大鼠体重增重显著增加,骨密度显著降低(P<0.05),肠道菌群多样性未见明显变化,厚壁菌门丰度降低,拟杆菌门丰度增加;与模型组相比,碳酸钙补充组大鼠体重增重显著降低,骨密度显著增加(P<0.05),肠道菌群多样性未见明显变化,厚壁菌门丰度增加,拟杆菌门丰度降低。结论实验周期内补钙能够升高大鼠骨密度,并抑制体重增加,其作用机制可能与调节肠道菌群结构有关。

Effects of calcium on the structure of intestinal microbiota in ovariectomized rats

OBJECTIVE To study the effect of calcium content on bone mineral density and intestinal microbiota in ovariectomized rats. METHODS A total of 30 Wistar rats were randomly divided into 3 groups, namely the sham operation group, the model group and the calcium carbonate supplement group. Postmenopausal osteoporosis rats were established by ovariectomy(OVX). After the first week of modeling, each group was intragastric separately. The calcium carbonate solution was given to the calcium carbonate supplement group, and the same amount of sterile water was given to the other two groups.The animals were executed at the end of the 12 weeks. Left femur was taken and bone mineral density(BMD) was detected by dual-energy X-ray absorptiometry. The total DNA of fecal samples was aseptically extracted and the Illumina Miseq platform was to carry out high-throughput sequencing. RESULTS Compared with the sham operation group, the body mass increment of rats in the model group was significantly increased, the level of BMD was significantly reduced(P<0.05), the intestinal microbiota diversity was not significantly changed, the Firmicutes abundance was decreased, and the Bacteroidetes abundance was increased. Compared with the model group, the body mass increment of rats in the calcium carbonate supplementation group was significantly reduced and BMD was significantly increased(P<0.05). There was no significant change in the intestinal microbiota diversity, Firmicutes were increased, and Bacteroidetes were decreased. CONCLUSION Calcium supplementation can improve bone mineral density and inhibit the increase of body mass during the experiment period, and its mechanism may be related to regulating the structure of intestinal microbiota.