润喉清咽口崩片对急性咽炎动物模型的药效学研究

目的:探讨润喉清咽口崩片与原剂型润喉清咽合剂对大鼠急性咽炎模型的治疗作用。方法:采用咽部氨水喷雾方法建立大鼠急性咽炎模型,随机分为正常组、模型组、润喉清咽合剂组、润喉清咽口崩片组,给予对应干预后,观察大鼠咽部组织病理改变,相关炎性因子的改变;通过小鼠模型分析其抗炎及镇痛作用。结果:润喉清咽口崩片和合剂型均可改善大鼠咽部病理改变,显著减少大鼠血清中的白细胞计数、中性粒细胞及淋巴、单核细胞数量(P<0.05),降低IL-1β、IL-6、TNF-α含量(P<0.05);提高小鼠30min、120min热刺激痛阈值(P<0.05),抑制小鼠耳肿胀(P<0.05),实验发现口崩片与合剂的治疗效果相当。结论:润喉清咽口崩片对急性咽炎具有较好的治疗作用,调节炎性反应可能是其主要作用机制,改变润喉清咽合剂剂型为口崩片具有进一步研究和推广价值。

Pharmacodynamic study of Runhou Qingyan Oral Disintegration Tablet on acute pharyngitis animal model

Objective: To explore the therapeutic effect of Runhou Qingyan Oral Disintegration Tablet(RQOD) and the original dosage form of Runyan Qingyan Mixture(RQM) on acute pharyngitis model in rats. Methods: The model of acute pharyngitis was established by aqueous ammonia spray method, rats were divided into normal control group, model group, mixture group, oral disintegrating group after model establishing. After the intervention, the pathological changes of pharynx tissue and the changes of inflammatory factors were observed. Anti-inflammatory and analgesic effects were tested by mouse model. Results: Both RQOD and RQM could improve the pathological changes of rat pharynx, significantly reduce the total number of white blood cells, neutrophils, lymphocyte and monocyte in the serum of rats(P<0.05), and decreased the content of IL-1β、IL-6 and TNF-α(P<0.05), and significantly increased the 30 min, 120 min heat stimulation pain threshold(P<0.05), inhibited mouse ear swelling(P<0.05), and it was equivalent to the therapeutic effect of the mixture group. Conclusion: RQOD has a good therapeutic effect on acute pharyngitis, regulating the inflammatory reaction is the main mechanism, changing the dosage form has further research and popularization value.