热休克蛋白27在大肠癌中的表达及其与淋巴结转移的关系

目的 探讨热休克蛋白27(HSP27)表达与大肠癌淋巴结转移的关系.方法 应用免疫组化方法检测68例临床大肠癌组织标本中Hsp27的表达情况.应用RT-PCR、Western blotting和免疫组化方法检测不同转移潜能大肠癌细胞株中Hsp27 mRNA和蛋白的表达情况.结果 在大肠癌组织中,热休克蛋白27的表达率与年龄、性别、淋巴结转移、临床分期无相关性(χ2test,P＞0.05),但其过表达和大肠癌淋巴结转移显著负相关(Fisher's exact test,P=0.035).应用RT-PCR、Western blotting和免疫组化方法检测结果显示Hsp27基因和蛋白在高淋巴结转移潜能的SW620细胞中呈低表达,在低淋巴结转移潜能的SW480细胞中呈高表达.结论 Hsp27和大肠癌肿瘤细胞的转移行为可能为负相关,可能在阻止其转移中发挥重要作用.

Expression of HSP27 in colorectal carcinoma and its relationship with lymphatic metastasis

OBJECTIVE: To investigate the relationship between heat shock protein 27 (HSP27) expression and lymphatic metastasis of colorectal carcinoma (CRC). METHODS: Immunohistochemistry was used to detect HSP27 expression in 68 specimens of human CRC. The expression of HSP27 mRNA and protein was also detected in two colorectal carcinoma cell lines with different lymphatic metastasis potentials by RT-PCR, Western blotting and immunohistochemistry. RESULTS: HSP27 expression was not related to such clinicopathological factors as the patient's age, gender, histological grade, lymphatic metastasis or clinical stages (P>0.05), but overexpression of HSP27 showed significant inverse correlation to lymphatic metastasis of CRC (P=0.035). HSP27 mRNA and protein were expressed at low levels in SW620 cells with high potentials for lymphatic metastasis, and at high levels in SW480 cells that had low lymphatic metastasis potentials. CONCLUSION: HSP27 expression can be inversely correlated to metastatic behavior of CRC cells, and may play a role in suppressing the metastasis of CRC.