银杏内酯B的提取、分离与纯化研究

目的:建立1种较简单的从银杏叶中提取、分离和纯化银杏内酯B的方法。方法:以银杏内酯B得率为指标,对银杏内酯B的提取、分离和纯化工艺进行优选。结果:银杏叶用70%乙醇提取3次,浓缩至无醇味,加水稀释至每1mL含生药材0.1g,HPD-450大孔吸附树脂吸附,20%乙醇除杂,80%乙醇洗脱、浓缩、结晶,异丙醇重结晶,得到纯度为95%的银杏内酯B结晶。结论:该工艺稳定、简便易行,适合工业化大生产。

Extraction, isolation and purification for Ginkgolide B

Objective : To establish a simple extraction, isolation and purification method for Gingkolide B from Ginkgo leaf. Method : The optimum conditions of extraction, isolation and purification were studied by taking the transfer rate of Ginkgolide B as index. Result : Ginkgo leaf was extracted with 70% ethanol for three times, the extracts were concentrated to remove ethanol and diluted by water till the crude drug density reached 0.1 g·mL^-1. The dilution was adsorbed with HPD-450 macroporous resin. The impurities were eluted with 20% ethanol and Ginkgolide B was eluted with 80% ethanol. Then the 80% ethanol eluant was concentrated and crystallized. Finally the crude crystals were recrystallized with isopropanol. The purity of the Ginkgolide B recrystallization was 95%. Conclusion : The process was stable and easy to operate, which was suited to industrialized production.