| DNA TYPING SYSTEM FOR HLA-A2 ALLELES BY POLYMERASE CHAIN REACTION WITH SEQUENCE-SPECIFIC PRIMERS |
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| 引用本文： | 张庆瑞 翟宁 耿龙 宋芳吉. DNA TYPING SYSTEM FOR HLA-A2 ALLELES BY POLYMERASE CHAIN REACTION WITH SEQUENCE-SPECIFIC PRIMERS[J]. 中国医学科学杂志(英文版), 2001, 16(3): 161-164 |
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| 作者姓名： | 张庆瑞 翟宁 耿龙 宋芳吉 |
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| 作者单位： | [1]DevartmentofDermatology.the202HospitalofPLA,Shenvang110003 [2]KeyLaboratoryofImmunodermatology,theFirstHospitalofChinaMedicalUniversity,Shenyang110001 |
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| 摘 要： | Objectivs. To establish a PCR-SSP method for discriminating as many HLA-A 02 alleles, which could easilybe introduced into a routine laboratory. Methods. In this study we typed HLA-A 02 polymorphisms by a sequence-specific primer (SSP) method,which involved round 1 and round 2 PCR reactions to detect 17 HLA-A 02 alleles (they are HLA-A 0201- 0217 alleles) covering exon 2 and exon 3. Results. We have fmmd that DNA sample concentration and purity were the most important variables in determin-ing the quality of the results. For identiffing correct band size, the size marker used was important. We noticed that different PCR machines pedormed differently. By this method, we detected 20 HLA-A 02 positive genomic DNA samples and found 4 kinds of HLA-A 02 alleles. They were HLA-A 0201, 0203, 0206 and 0210. Condusion. The HLA-A 02 PCR-SSP method was proven to be a reliable and easily applicable typing method. Our results suggest that the SSP described here provides an optimal HLA-A 02 typing technique that may be useful in selecting donor-recipient pairs in bone marrow transplantation between unrelated individuals.
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| 关 键 词： | 脱氧核糖核酸类型 人白细胞抗原 等位基因 聚合酶链反应 PCR-SSP HLA-A2 |
DNA TYPING SYSTEM FOR HLA-A2 ALLELES BY POLYMERASE CHAIN REACTION WITH SEQUENCE-SPECIFIC PRIMERS |
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| Zhang Qingrui , Zhai Ning, Geng Long and Song Fangji. DNA TYPING SYSTEM FOR HLA-A2 ALLELES BY POLYMERASE CHAIN REACTION WITH SEQUENCE-SPECIFIC PRIMERS[J]. Chinese medical sciences journal, 2001, 16(3): 161-164 |
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| Authors: | Zhang Qingrui Zhai Ning Geng Long Song Fangji |
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| Affiliation: | Department of Dermatology, the 202 Hospital of PLA, Shenyang 110003. |
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| Abstract: | Objective. To establish a PCR- SSP method for discriminating as many HLA- A* 02 alleles, which could easily be introduced into a routine laboratory. Methods. In this study we typed HLA- A* 02 polymorphisms by a sequence- specific primer (SSP) method, which involved round 1 and round 2 PCR reactions to detect 17 HLA- A* 02 alleles (they are HLA- A* 0201- 0217 alleles) covering exon 2 and exon 3. Results. We have found that DNA sample concentration and purity were the most important variables in determining the quality of the results. For identifying correct band size, the size marker used was important. We noticed that different PCR machines performed differently. By this method, we detected 20 HLA- A* 02 positive genomic DNA samples and found 4 kinds of HLA- A* 02 alleles. They were HLA- A* 0201, 0203, 0206 and 0210. Conclusion. The HLA- A* 02 PCR- SSP method was proven to be a reliable and easily applicable typing method. Our results suggest that the SSP described here provides an optimal HLA- A* 02 typing technique that may be useful in selecting donor- recipient pairs in bone marrow transplantation between unrelated individuals. |
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| Keywords: | DNA typing PCR- SSP HLA- A* 02 alleles |
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