胎盘小叶胎儿血管系统CRH及其受体CRH-R1的表达

目的 了解足月胎盘小叶胎儿血管系统促肾上腺皮质激素释放激素（CRH）及其受体（CRH-R1）的表达。方法 分别采用免疫组织化学技术LsAB法和原位杂交技术，观察先兆临产前人足月正常胎盘小叶血管CRH及其受体CRH-R1的表达。结果 胎盘小叶血管内皮细胞、血管平滑肌细胞的细胞浆内均有CRH／CRH-R1阳性表达，同时也有CRH mRNA／CRH-R1 mRNA阳性表达。结论 胎盘小叶脐血管系统血管内皮细胞甚至平滑肌细胞能合成CRH及CRH-R1，其CRH可能是胎儿血CRH的主要来源之一。

Expression of Corticotropin-releasing Hormone and Its Receptor Type-1 in the Placental Cotyledon Vessels

Objective To study the expression of corticotropin-releasing hormone(CRH) and its receptor type-1 in the placental cotyledon vessels at term. Methods IHC-SP staining and ISH methods were used to investigate the expression patterns of CRH and its receptor CRH-R1 in the placental cotyledon vessels at term from women who had undergone uncomplicated pregnancies. Placental tissues were collected after cesarian section before threatened labor. Results This study revealed for the first time that endothelial cells and even smooth muscle cells of the cotyledon vessel expressed CRH messenger ribonucleic acid and peptide as well as the CRH-R1. Conclusion The novel findings disclosed that the CRH derived from endothelial cells and even smooth muscle cells of the cotyledon vessel may represent the main source of CRH levels in fetal plasma, thus indicating the capability of mobilizing the compensation mechanism for adapting to the acute stress.