垂体腺瘤染色体改变与侵袭性的相关性

目的研究垂体腺瘤染色体改变及其与肿瘤临床侵袭性的相关性,进一步了解垂体腺瘤生物学行为转变的细胞遗传学基础.方法将30例经蝶窦垂体腺瘤切除手术中取得的新鲜垂体腺瘤组织制作单细胞悬液,体外直接阻滞法获取染色体,结合R显带核型分析和间期荧光原位杂交技术(inter-phase FISH),对染色体变异进行观察、分析.结果垂体腺瘤染色体变异主要为数目改变,涉及除4、16、Y之外的每一条染色体.侵袭性垂体腺瘤的染色体变异几率高于非侵袭性垂体腺瘤.8、9、12、19号染色体的增加在垂体腺瘤染色体变异中十分常见, 9、11、19号染色体变异和8号四体,9号四体多见于侵袭性垂体腺瘤.间期FISH验证并补充了常规核型分析的结果.结论侵袭性垂体腺瘤的染色体变异几率高于非侵袭性垂体腺瘤.8、12号染色体的改变可能在垂体腺瘤的发生起着重要作用,而9、11和19号染色体的改变可能与肿瘤侵袭性的增加相关.

The relation of chromosome abnormality to tumor invasiveness in pituitary adenomas

Objective To study the chromosome abnormality and the relation to invasiveness of pituitary adenomas.Methods Conventional R-band cytogenetics karyotype analysis and inter-phase fluorescence in situ hybridization(FISH)were used to test 30 specimens of pituitary adenoma.Results The chromosome numeric variance involved chromosomes except 4, 16 and Y, having significant difference between invasive pituitary adenomas and noninvasive ones. The increase of 8, 9, 12, 19 chromosomes was more frequent. The aberration of 9, 11, 19 chromosomes and 8, 9 tetrasome were found to be more often presented in invasive pituitary adenomas. Conclusion The chromosome numeric alteration is quite different in invasive and noninvasive pituitary adenomas. 8, 12 chromosomes may relate to the occurrence of pituitary adenoma, and 9, 11, 19 chromosomes may cause the change of pituitary adenomas invasiveness.