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多重巢式RT-PCR技术在急性髓系白血病中的应用
引用本文:赵瑾,周永安,苏丽萍,武坚锐,王恺,解菊芬,马莉. 多重巢式RT-PCR技术在急性髓系白血病中的应用[J]. 白血病.淋巴瘤, 2002, 18(1): 277-280. DOI: 10.3760/cma.j.issn.1009-9921.2009.05.008
作者姓名:赵瑾  周永安  苏丽萍  武坚锐  王恺  解菊芬  马莉
作者单位:山西医科大学研究生处,太原,030001;山西省儿童医院检验科;山西省肿瘤医院血液科;
摘    要:Objective To analyse the fusion genes derived from chromosome structural aberrations in acute myeloid leukemia(AML) and the relationship between fusion genes and the MICM classification, clinical diagnosis, chemotherapy and prognosis. Methods The expression of fusion gene in bone marrow samples was detected with multiplex RT-PCR technique and chromosome karyotypes, immunological phenotypes and clinical data were analyzed in 60 acute myeloid leukemia newly diagnosed. Results 37 cases(61.67 %) of 60 patients carried 5 kinds of fusion genes consisting of MLL-AF9, TLS-ERG, CBFβ-MYH1, AML1-ETO and PML-RARα. The activation of oncogene HOX11 was detected in 13 AML cases, three of them with other chromosome aberration simultaneously.23 cases of 31 patients carrying AML1-ETO or PML-RARα, reached complete remission(CR) after chemotherapy and without relapse. Conclusion Gene typing is the most precise classification method that can direct clinical treatment and evaluate prognosis. Multiplex RT-PCR technique, which can quickly screen 29 kinds of fusion gene derived from chromosome structural aberrations at one time, maybe helpful to improve M1CM classification and guide the choice of treatment.

关 键 词:急性髓系白血病   染色体结构畸变   融合基因   多重RT-PCR   

Application of multiplex reverse transcription polymeruse chain reaction in acute myeloid leukemia
ZHAO Jin,ZHOU Yon-gan,SU Li-ping,WU Jian-rui,WANG Kai,XIE Ju-fen,MA Li. Application of multiplex reverse transcription polymeruse chain reaction in acute myeloid leukemia[J]. Journal of Leukemia & Lymphoma, 2002, 18(1): 277-280. DOI: 10.3760/cma.j.issn.1009-9921.2009.05.008
Authors:ZHAO Jin  ZHOU Yon-gan  SU Li-ping  WU Jian-rui  WANG Kai  XIE Ju-fen  MA Li
Abstract:
Keywords:Acute myeloid leukemiaChromosomal structural aberrationFusion geneMultiplex RT-PCR
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