银屑病患者血浆和皮损纤维连接蛋白含量测定及分子降解状态的研究

为了探讨银屑病患者血浆纤维连接蛋白（ＰＦＮ）和皮损可溶性纤维连接蛋白（ＳＦＮ）的存在状态，采用火箭电泳法和蛋白印迹法分别对９０例银屑病ＰＦＮ和２０例银屑病斑块状皮损ＳＦＮ进行检测。结果表明，患者组ＰＦＮ水平显著低于正常人对照组，皮损ＳＦＮ含量明显高于正常人皮肤。扫描结果提示，患者组ＰＦＮ降解增强可能是ＰＦＮ减少的主要原因，不同期银屑病组ＰＦＮ降解程度有差异。皮损ＳＦＮ增多可能是由于局部合成增加及ＰＦＮ向表皮渗漏所致。动态观察银屑病患者ＰＦＮ分子降解状态，具有重要的临床意义

Determination of Plasma and Lesional Fibronectin and Study on the Status of Molecular Degradation in Patients with Psoriasis

In order to study the status of existence of plasma fibronectin (PFN) and lesional soluble fibronectin (SFN) in patients with psoriasis, the levels of PFN and lesional SFN were detected by rockect electrophoresis and Western blot in 90 patients with psoriasis vulgaris and 20 patients with plaque psoriasis respectively. The results showed that in comparison with the control group, the level of PFN in psoriasis group decreased significantly; the content of SFN in psoriatic plaque was significantly higher than that in the normal human skin. Scanning (scanning densitometer) results indicated that the enhanced degradation of PFN in psoriatic group might be the main reason of reduced level of PFN. The extent of PFN degradation was different in different stages of the disease in psoriatic group. The increase of lesional SFN might be caused by increased local fibronectin synthesis and effusing of PFN into the epidermis. Dynamic observation of PFN molecular degradation may be of important clinical significance in patients with psoriasis.