喉癌组织炎症细胞因子和受体相关基因的表达谱

目的研究喉癌组织炎症细胞因子和受体相关基因的表达谱,探讨相关基因在喉癌发病机制和肿瘤免疫中的作用。方法分别抽提2例喉癌组织和癌旁组织的总RNA,逆转录成互补DNA(complementary DNA,cDNA)一链,合成cRNA,采用生物素标记制备成杂交探针,在4张炎症细胞因子和受体相关基因芯片上进行杂交,并用化学发光检测,数字化处理和分析。结果喉癌组织组织炎症相关基因表达谱中差异表达的基因共40条,其中上调基因22条,下调基因18条;2张芯片共存的差异基因13条,其中上调基因10条,下调基因3条。结论喉癌组织炎症细胞因子和受体相关基因为肿瘤与炎症相关性研究,以及肿瘤免疫学机制研究提供线索和理论依据。其中CCL7可能在喉癌的发生和发展中发挥一定的作用,肿瘤获得性免疫的相关机制还需要进一步研究。

Expression profile of inflammation related genes in laryngeal cancer

OBJECTIVE To investigate the expression profile of inflammation related genes in laryngeal squamous cell carcinoma by functional gene chip technique and to probe into the role of correlative genes in pathogenesis of the laryngeal squamous cell cancer and in tumor immunity. METHODS The total RNAs were respectively extracted from two pair samples of laryngeal tumor and the normal tissue around the tumor, and then were reversely transcribed to cDNAs, then synthesized to cRNAs. The cRNAs were labeled with the hybridization probes. The probes were then hybridized with four pieces of inflammation related genes chip. It was chemiluminescently detected and the acquired image was analyzed with special software. RESULTS Forty genes were differently expressed in inflammation related gene profile of laryngeal tumor, among which 22 genes were upregulated and 18 genes were down regulated. Thirteen genes were shown differential expression in both chips with 10 upregulated genes and 3 downregulated genes. CONCLUSION The differently expressed genes in inflammation related gene chips will provide clues and theoretical foundation for the investigation of the relationship between tumor and inflammation, and also the immune pathogenesis of laryngeal tumor. Furthermore CCL-7 may have an important role in the occurrence of laryngeal cancer, and the role of immunity of the pathogenesis of laryngeal tumor needs further studied.