降钙素基因相关肽对血管平滑肌表型转化和增殖的影响

目的 探讨降钙素基因相关肽(CGRP)对大鼠血管平滑肌细胞(VSMCs)表型转化和增殖的影响以及它们之间的关系.方法 取大鼠主动脉先体外培养8d后再贴块培养(VSMCs);对照组直接用贴块法培养细胞.实验分为CGRP作用组和无CGRP作用组.用5-BrdU标记平滑肌细胞增殖变化;RT-PCR检测HRG-1和SM22α表达变化.结果 血管经体外培养8d后再贴壁培养的平滑肌细胞可见大量棕黄色标记增殖的细胞核,HRG-1和SM22α mRNA表达明显减少;而CGRP作用组标记的血管平滑肌增殖细胞明显减少,HRG-1和SM22α mRNA表达明显上调.结论 CGRP对VSMCs增殖有抑制作用并同时可使VSMCs从合成型向收缩型转化.

INFLUENCE OF CALCITONIN GENE-RELATED PEPTIDE ON THE PROLIFERATION AND PHENOTYPE TRANSFORMATION OF VASCULAR SMOOTH MUSCLE CELLS

Objective To investigate whether calcitonin gene related peptide (CGRP) can influence the proliferation and phenotypic modulation of vascular smooth muscle cells(VSMCs), and what is the relationship between them. Methods Vascular smooth muscle cells(VSMCs) were cultured respectively with rat aorta cultivated for 8 days in vitro and with normal aorta (not culture) through the explant attached method, and CGRP was added into the culture medium of the experimental groups. The proliferation of cells was labeled by 5 bromodeoxyuridine (5 BrdU) with immunocytochemical method, and the mRNA expression of hypertension related gene 1 (HRG-1) and smooth muscle 22 alpha (SM22α) were determined by RT-PCR. Results The proliferating cells labeled by BrDU from the aorta cultured for 8 days in vitro were increased notablly and the mRNA expression of HRG 1 and SM22α were decreased. While the VSMCs were cultured in the culture medium containing CGRP, the proliferous cells labeled by BrdU were obviously decreased and the mRNA expression of HRG-1 and SM22α were significantly increased. Conclusion It is showed that CGRP could inhibit the proliferation of VSMCs and change the phenotype of