成年牦牛松果体的光镜和电镜观察

目的 探讨成年牦牛松果体形态结构特征,为哺乳动物松果体形态结构研究积累资料.方法 光镜HE染色、浸银染色和透射电子显微镜技术.结果 光镜下,牦牛松果体由松果体细胞、少量的神经胶质细胞、毛细血管和神经等组成.电镜下,松果体细胞电子致密度低,细胞质内含丰富的线粒体、粗面内质网、滑面内质网、微管、微丝和核糖体;高尔基复合体数量极少,典型异质细胞器突触带呈球形,多位于质膜附近.神经胶质细胞的细胞质内含丰富的线粒体,其胞体突起呈球形膨大伸入到松果体细胞之间.松果体细胞以及神经胶质细胞间均存在突触和连接复合体.牦牛松果体内毛细血管为连续型,其远腹侧血管周围可见色素细胞.结论 成年牦牛松果体细胞内存在神经上皮样和腺上皮样2种细胞连接方式.毛细血管为连续型.松果体细胞内细胞器发达,但很少观察到高尔基复合体.

OBSERVATION OF ADULT YAK PINEAL GLAND BY LIGHT AND ELECTRON MICROSCOPY

Objective To study the characteristic of histological structure of the pineal gland of the adult yak. Methods HE and Achucarro-Hortega staining,light microscopy and transmission electron microscopy were used. Results The pineal gland was composed of pinealocytes,occasional glial cells,capillaries and neural elements.The pinealocyte appppered clear electronic density and its cytoplast included numerous mitochondria,rough and smooth endoplasmic reticulum,microtubules,microfilament and ribosome.Golgi complex was rare.The typical heterogenous organelle of pinealocyte of yak was spherical synaptic ribbon,which located near the plasma membrane.The glial cells contained abundant of mitochondria,and their processes which appeared bulbous invested the pineal periphery and incompletely separated the pinealocytes.Synapse and junctional complex existed between the pinealocytes and glial cells.Capillaries in pineal gland of the yak were belong to continuous capillaries,and pigment cells were observed around the vascellum in the gastr-distally of the pineal gland. Conclusion Neuroepithelial and epithelioglandular cell conjuction can be observed in the pineal gland of adult yak.Capillaries are belong to continuous capillaries.There are aboundant of organelle except Golgi complex in the pinealocyte.