养血清脑颗粒对蒙古沙鼠全脑缺血再灌注后海马CA1区神经元的治疗作用

目的 探讨蒙古沙鼠全脑缺血再灌注后养血清脑颗粒(Yangxueqingnaokeli,YXQNKL)的治疗作用.方法 采用蒙古沙鼠两侧颈总动脉结扎法,缺血30min再灌注5d模型(分为假手术组、缺血再灌注组、养血清脑颗粒治疗组).用Nissl染色法观察海马CA1区神经元的形态和数量,用免疫组织化学方法观察海马CA1区神经元神经钙离子感应蛋白1(NCS-1)、天冬氨酸特异性半胱氨酸蛋白酶3(caspase-3)和谷氨酸盐合成酶(Glusyn)的表达情况.结果 与缺血再灌注组比较,缺血再灌注 0.4g/kg养血清脑颗粒治疗组和缺血再灌注 0.8g/kg养血清脑颗粒治疗组Nissl染色显示海马CA1区神经元数量明显增加(P＜0.05);免疫组织化学法显示海马CA1区神经元细胞NCS-1、caspase-3和Glusyn的阳性细胞数明显减少(P＜0.05).结论 全脑缺血再灌注后,给予养血清脑颗粒能显著增加海马CA1区神经元数量,这与其减少海马CA1区神经元NCS-1、caspase-3和Glusyn的表达有密切的联系.

THE THERAPEUTICAL EFFECTS OF YANGXUEQINGNAOKELI ON HIPPOCAMPUS CA1 NEURONS IN MONGOLIAN GERBILS AFTER GLOBAL ISCHEMIC REPERFUSION INJURY

Objective To investigate the therapeutical effects of Yangxueqingnaokeli(YXQNKL) on global ischemia reperfusion injury in Mongolian gerbils. Methods The global cerebral ischemia of gerbils was induced by clamping bilateral carotid arteries for 30 minutes and reperfused for 5 days.Nissl staining was employed to observe the morphology and numbers of the gerbils' CA1 hippocampal neurons,and immunohistochemical stainings of Glusyn,NCS-1 and caspase-3 were used to observe their expressions in the gerbils' CA1 hippocampal neurons. Results Nissl staining showed that the CA1 hippocampal neurons both in I/R 0.4g/kg YXQNKL group and I/R 0.8g/kg YXQNKL group were much more than those in the I/R group(P<0.05),and immunohistochemical stainings indicated that the positive cells of NCS-1 and caspase-3,Glusyn in CA1 hippocampal neurons were significantly decreased compared with those in the I/R group(P<0.05).Conclusion Treatment with YXQ can increase the living neurons in hippocampus CA1 region potently against global ischemia reperfusion brain injury,which is closely related to the decreased expression of NCS-1,caspase-3 and Glusyn.