养血清脑颗粒对蒙古沙鼠全脑缺血再灌注后的神经保护作用

目的 探讨养血清脑颗粒对蒙古沙鼠全脑缺血再灌注后的神经保护作用.方法 用两血管阻塞法(2-VO)结扎30 min再灌注损伤模型,将蒙古沙鼠随机分为假手术组、模型组和手术前90min灌胃法给药预防组.用Nissl染色法观察蒙古沙鼠海马CA1区细胞的变化;用免疫组织化学方法观察海马CA1区表达谷氨酸盐合成酶(G1 Syn)和Caspase-3阳性细胞数量的变化;TUNEL法检测细胞凋亡.结果 蒙古沙鼠全脑缺血再灌注1d及2d预防给药组Nissl染色结果显示,海马CA1区存活细胞数量比模型组明显增多(分别为P＜0.01,P＜0.05);再灌注5d预防给药组海马CA1区存活细胞数量与模型组相比无显著性差异(P＞0.05).再灌注1d及2d预防给药组海马CA1区表达谷氨酸盐合成酶(G1 Syn)的阳性细胞量较模型组明显减少(分别为P＜0.01,P＜0.05);再灌注5d预防给药组海马CA1区免疫阳性细胞数量与模型组相比无显著性差异(P＞0.05);Caspase-3的表达在再灌注1d时预防给药组免疫阳性细胞数量与模型组比较有显著性升高(P＜0.05),但是再灌注2d及5d时预防给药组免疫阳性细胞数较模型组无显著降低(P＞0.05).TUNEL结果显示预防给药组凋亡细胞相应减少.结论 养血清脑颗粒能通过谷氨酸盐合成酶选择性抑制兴奋性氨基酸谷氨酸的神经毒性作用,减少神经细胞的凋亡,从而发挥神经保护作用.

THE NEURAL PROTECTION OF YANGXUEQINGNAOKELI IN GLOBAL CEREBRAL ISCHEMIA AND REPERFUSION GERBILS

Objective To study the neural protection of Yangxueqingnaokeli in global ischemia and reperfusion gerbils.Method Gerbils were randomly divided into sham group,global ischemia group and global ischemia treated with Yangxueqingnaokeli group.The ischemic model was performed by occlusion of 2-VO for 30minutes with reperfusion.The sections were stained by Nissl and immunohistochemistry staining and the data were analysed with SAS soft ware.Results The quantity of the surviving cells in CA1 region of hippocampus increased significantly in the group reperfused for 1 or 2days with Yangxueqingnaokeli treatment compared with the global ischemia group(P<0.01,P<0.05,respectively),but without significant difference between the group reperfused for 5days and the group treated with Yangxueqingnaokeli(P>0.05).The expression of glutamate synthetase in the hippocampus was significantly reduced in the group reperfused for 1 or 2days with Yangxueqingnaokeli treatment compared with the global ischemia group(P<0.01,P<0.05,respectively),without significant difference between the group reperfused for 5days and the global ischemia group(P>0.05),meanwhile the expression of Caspase-3 in the hippocampus was significantly reduced in the group reperfused for 1day with Yangxueqingnaokeli treatment compared with the global ischemia group(P<0.05),without significant difference between the group reperfused for 2 or 5days and the global ischemia group(P>0.05).The treatment also reduced the number of TUNEL-positive cells.Conclusion Yangxueqingnaokeli might selectively downregulate the expression of glutamate synthetase to reduce the number of apoptotic cells to play the neural protective effect.