大鼠肺挫伤白细胞介素17含量变化及L-精氨酸的影响

目的:了解白细胞介素17(IL-17)在大鼠创伤性肺挫伤中的含量及意义,并观察L-精氨酸的影响。方法:60只SD大鼠随机分为正常组、模型组和L-精氨酸组,每组20只。采用胸部撞击器制备肺挫伤模型。L-精氨酸组,模型制备后经股静脉给予L-精氨酸(250mg/kg),各组建立模型后分别于24h处死动物并收集标本。酶联免疫吸附法(ELISA)测定肺组织匀浆及支气管肺泡灌洗液(BALF)中IL-17含量。测定肺组织髓过氧化物酶(MPO)活性。结果:与对照组比较,模型组大鼠肺组织匀浆及BALF中IL-17含量均显著升高(P<0.01);模型组肺组织IL-17、肺组织髓过氧化物酶(MPO)活性与L-精氨酸组比较明显偏高(P<0.05)。结论:IL-17在大鼠肺挫伤中显著升高。L-精氨酸治疗创伤性肺挫伤,能明显下调肺组织IL-17、肺组织髓过氧化物酶(MPO)活性,改善肺组织微循环,减轻炎症反应。

Changes of interleukin-17 and effects of L-Arginine on rats lung with traumatic pulmonary contusion

Objective:To investigate the changes of interleukin-17(IL-17) and the effects of L-arginine on traumatic pulmonary contusion.Method:Sixty Sprague-Dawley rats were randomly divided into three groups,i.e.normal group,model group,and L-arginine group.The model of traumatic pulmonary contusion was established with chest-impacter.Then the rats in the L-arginine group were injected intravenously with L-arginine in a dose of 250 mg/kg.All rats were sacrificed at 24 hours after the models were established.ELISA was adopted to detect the levels of IL-17 in lung tissue homogenate as well as in bronchoalveolar lavage fluid(BALF).The activity of myeloperoxidase(MPO) in the lung tissue was measured.Result:The IL-17 levels in lung tissue homogenate as well as in BALF were higher in model group than those in the control group(all P<0.01).Compared with the model group,IL-17 and the activity of MPO in lung tissue decreased significantly in the L-arginine group(P<0.05).Conclusion:The expression of IL-17 increased in traumatic pulmonary contusion rats.L-arginine treatment can down-regulate the expression of IL-17,MPO,and ameliorate the microcirculation of rat’s lung with traumatic pulmonary contusion.