扶正化瘀方对肝纤维化大鼠肝脏明胶酶、TIMP-2和星状细胞凋亡的影响

为研究扶正化瘀方(主药：丹参、桃仁、人工虫草菌丝等)治疗肝纤维化的机理,60只Wistar雄性大鼠。除8只作为正常对照组(A,腹腔注射生理盐水)外,52只大鼠腹腔注射0.5％对二甲基亚硝胺(共4周,每周3次,每次2ml／kg),造成肝纤维化模型,并随机分为3组。模型对照组(B,18只)、扶正化瘀方组(C,17只)、秋水仙碱组(D,17只)。分别给予自来水以及相应中西药物灌胃,共4周。之后,处死大鼠取材检测各项指标。结果：C组大鼠Hyp和肝组织胶原分期下降。纤维化程度减轻;活化HSC减少。凋亡增加;肝组织MMP-2、MMP-9、TIMP-2表达下降。结论：促进活化HSC的凋亡、减少活化HSC的数量,并由此降低MMP-2、MMP-9、TIMP-2的表达、减轻肝纤维化程度,可能是扶正化瘀方的作用机理之一。

Influence of Health-Supporting and Stasis-Resolving Decoction on Gelatin Enzyme,TIMP-2 and Astrocyte Apoptosis in Rat Liver

To explore the mechanism of "Health-Supporting and Stasis-Resolving Decoction" in the treatment of liver fibrosis,52 male Wistar rats were given intra-abdominal injection of 0.5% p-dimethyl-nitroamine(2ml/2kg,three times a week for 4 weeks)to induce liver fibrosis,and then randomized into three groups:group B in which 18 rats were given tap water,group C in which 17 rats were given the Decoction and group D in which 17 rats were given colchicine;moreover,another 8 rats in group A were used as control.Four weeks later,all rats were sacrificed and relevant indexes were detected.Results:The Hyp and liver tissue collagen phase as well as fibrosis reduced;activated HSC decreased and apoptosis increased;the MMP-2,MMP-9 and TIMP-2 expressions reduced.Conclusion:The Decoction can promote the apoptosis of activated HSC and reduce activated HSC to reduce MMP-2,MMP-9 and TIMP-2 expressions,as a result to relieve liver fibrosis.