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Effect of antisense oligodeoxynucleotide of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer |
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| Authors: | Jiang Ying-An Luo He-Sheng Fan Li-Fang Jiang Chong-Qing Chen Wei-Jin |
| Institution: | 1. Department of Gastroenterology,Renming Hospital of Wuhan University, Wuhan 430060, Hubei Province, China 2. Department of Pathology, Medical College of Wuhan University, Wuhan 430071, Hubei Province, China 3. Department of General Surgery, Zhongnan Hospital of Wuhan University, Wuhan 430071, Hubei Province, China 4. Department of Medical Information, Central Hospital of Huangshi City, Huangshi 435000, Hubei Province, China |
| Abstract: | AIM: To explore the effect of antisense oligodeoxynucleotide (As-ODN) of telomerase RNA on telomerase activity and cell apoptosis in human colon cancer. METHODS: As-ODN was transfected into SW480 cells by liposomal transfection reagent. Telomerase activity of SW480 cells was examined by telomeric repeat amplification protocol (TRAP) and enzyme-linked immunosorbent assay (ELISA). Apoptosis was analyzed by morphology and flow cytometry. RESULTS: The telomerase activity in SW480 cells transfected with 1.0 micromol/L of As-ODN for 2-5 days, was significantly decreased in a time-dependent manner, and the cells underwent apoptosis. The missense ODN (Ms-ODN) and the control group transfected with SW480 cells did not show these changes. CONCLUSION: As-ODN can specifically inhibit the telomerase activity of SW480 cells and induce apoptosis. |
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