荧光探针杂交技术检测临床标本内结核分支杆菌的价值

目的　评价聚合酶链反应(PCR)荧光探针杂交技术(TaqMan技术)检测临床标本中结核分支杆菌的应用价值。方法 应用细菌学方法(涂片镜检和培养)及TaqMan法检测133份结核病患者痰标本,53份非结核呼吸系疾病患者痰标本。结果 细菌学方法检测结核病患者临床标本中结核分支杆菌阳性率为36.1%,TaqMan法阳性率为61.7%,高于细菌学检测法,经统计学处理,两者有显著性差异(P<0.05),用TaqMan法检测临床标本特异性为96.2%。结论 TaqMan技术将PCR扩增、荧光探针杂交及检测一体化,在单一管内完成,具有简便、快速、防污染、敏感性及特异性较高等优点,是结核病辅助诊断的有效方法之一。

Clinical value of fluorogenic probe hybridzation assay for detecting Mycobacterium tuberculosis in clinical specimens

Objective To evaluate the clinical value of PCR-fluorogenic probe hybridzation assay (TaqMan assay) for detecting Mycobacterium tuberculosis in clinical specimens.Methods The sputum specimens of 133 patients with pulmonary tuberculosis and 53 patients with respiratory system diseases other than tuberculosis were tested by bacteriology(smear and culture)and TaqMan assay.Results The sensitivities of bacteriology and TaqMan assay for detecting Mycobacterium tuberculosis in sputum specimens of patients with pulmonary tuberculosis were 36.1% and 61.7% respectively.The specificity of TaqMan assay in tested clincal specimens was 96.2%.Conclusion The TaqMan assay performed in a hermetially sealed tube is simple,rapid ,anticontaminative, sensitive and specific. It is one of the effective methods for the diagnosis of pulmonary tuberculosis.