内衬上皮的管状组织工程软骨构建的实验研究

目的研究以聚丙交酯-乙交酯共聚物[poly（DL-lactide-co-glycolide）,PLGA]包埋甲壳胺无纺布为细胞支架,在有免疫力的动物体内构建内衬上皮的管状组织工程软骨的可行性,为喉、气管缺损的修复探索新方法。方法取1月龄兔耳廓软骨,收集体外培养第3～4代的软骨细胞,接种于经多聚赖氨酸处理的以PLGA包埋甲壳胺无纺布的片状细胞支架材料上,体外培养7～10d后包裹于兔腹部缝制的皮管上并深埋于体内,分别于6、12、18周取材行大体和组织学观察以及HE染色、甲苯胺蓝染色和Masson＇s三色染色。结果同种异体软骨细胞/PLGA包埋甲壳胺无纺布的细胞支架复合物体外培养1周时有基质产生;植入体内6周后,复合物中有基质分泌,软骨细胞不成熟;12周时,软骨细胞接近成熟并形成凹陷,内衬上皮的管状组织工程软骨形成;18周时,新生软骨基本接近正常软骨。结论以PLGA包埋甲壳胺无纺布为细胞支架,同种异体软骨细胞与细胞支架所形成的复合物在有免疫力的动物体内可构建出内衬上皮的管状组织工程软骨。

Fabrication of Allogenic Cartilage in A Tube Lined with Epithelium and A Novel Scaffold

Objective To study the feasibility of engineering cartilage tissue in a tube lined with epithelium and implanting allogenic chondrocytes into a novel scaffold consisting of chitosan nonwoven cloth coated with poly (DL-lactide-co-glycolide) (PLGA). The focus of the present study is to explore a new way of repairing laryngeal and tracheal defects. Methods Allogenic chondrocytes were obtained from the auricles of 1-month-old rabbits. After being cultured in vitro for three to four passages,the cells were impla...