海拔高度对蒙古黄芪主要药效成分积累及其关键酶基因表达量影响研究

目的 研究不同海拔高度与蒙古黄芪Astragalus membranaceus var.mongholicus主要药效成分含量及其关键酶基因表达量之间的关系.方法 采用HPLC法检测黄芪根中黄芪甲苷与毛蕊异黄酮葡萄糖苷的含量,采用实时荧光定量PCR法检测黄芪甲苷生物合成过程中关键酶基因乙酰辅酶A乙酰基转移酶[AACT,...

Study on effect of altitude on accumulation of main active components and expression of key enzyme genes of Astragalus mongholicus

Objective Astragalus membranaceus was used as the experimental material to study the relationship between different altitude and the content of main effective components and the expression of key enzyme genes of A. membranaceus. Methods The content of astragaloside and genistein glucoside in Astragalus root was detected by HPLC, and the expression of key enzyme genes (AACT, HMGR, CAS, FPS, HMGs, IDI, SE, SS) in astragaloside biosynthesis was detected by qRT-PCR. Results The contents of astragaloside a, isoflavone glucoside, and total saponin in HQ-2 of four plots in Huhhot were higher than those in other plots. The correlation analysis showed that the altitude had a great influence on the synthesis of astragalus total saponins, and the genes of IDI, SE and SS played a major role in the synthesis of astragalus total saponins. Altitude was favorable for the expression of IDI, SE and SS genes, and it also had a certain regulatory effect on key enzyme genes in astragaloside synthesis pathway. Conclusion Planting A. mongholicus at an altitude of 1730 meters is beneficial to the accumulation of its active components.