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Antigenic variation of H1N1, H1N2 and H3N2 swine influenza viruses in Japan and Vietnam |
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| Authors: | Nobuhiro Takemae Tung Nguyen Long Thanh Ngo Yasuaki Hiromoto Yuko Uchida Vu Phong Pham Tsutomu Kageyama Shizuko Kasuo Shinichi Shimada Yasutaka Yamashita Kaoru Goto Hideyuki Kubo Vu Tri Le Hung Van Vo Hoa Thi Do Dang Hoang Nguyen Tsuyoshi Hayashi Aya Matsuu Takehiko Saito |
| Institution: | 1. Thailand-Japan Zoonotic Diseases Collaboration Center, Kasetklang, Chatuchak, Bangkok, 10900, Thailand 2. Viral Disease and Epidemiology Research Division, National Institute of Animal Health, National Agriculture and Food Research Organization (NARO), 3-1-5 Kannondai, Tsukuba, Ibaraki, 305-0856, Japan 3. National Centre for Veterinary Diagnostics, 11/78 Giai Phong, Phuong Mai, Dong Da, Hanoi, Vietnam 4. Center for Veterinary Diagnostics, Regional Animal Health Office No. 6, 521/ 1 Hoang Van Thu St., Ward 4, Tan Binh District, Ho Chi Minh City, Vietnam 5. Influenza Virus Research Center, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashi-Murayama, Tokyo, 208-0011, Japan 6. Nagano Environmental Conservation Research Institute, 1978 Amorikomemura, Nagano, Nagano, 380-0944, Japan 7. Saitama Institute of Public Health, 639-1 Kamiokubo, Sakura-ku, Saitama, Saitama, 338-0824, Japan 8. Ehime Prefectural Institute of Public Health and Environmental Science, 8-234 Sanbancho, Matsuyama, Ehime, 790-0003, Japan 9. Osaka City Institute of Public Health and Environmental Sciences, 8-34 Tojocho, Tennouji-ku, Osaka, Osaka, 543-0026, Japan 10. The United Graduate School of Veterinary Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1193, Japan |
| Abstract: | The antigenicity of the influenza A virus hemagglutinin is responsible for vaccine efficacy in protecting pigs against swine influenza virus (SIV) infection. However, the antigenicity of SIV strains currently circulating in Japan and Vietnam has not been well characterized. We examined the antigenicity of classical H1 SIVs, pandemic A(H1N1)2009 (A(H1N1)pdm09) viruses, and seasonal human-lineage SIVs isolated in Japan and Vietnam. A hemagglutination inhibition (HI) assay was used to determine antigenic differences that differentiate the recent Japanese H1N2 and H3N2 SIVs from the H1N1 and H3N2 domestic vaccine strains. Minor antigenic variation between pig A(H1N1)pdm09 viruses was evident by HI assay using 13 mAbs raised against homologous virus. A Vietnamese H1N2 SIV, whose H1 gene originated from a human strain in the mid-2000s, reacted poorly with post-infection ferret serum against human vaccine strains from 2000-2010. These results provide useful information for selection of optimal strains for SIV vaccine production. |
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