叶绿素铜钠盐对AA模型基因表达谱的实验研究

目的探讨叶绿素铜钠盐治疗再生障碍性贫血(AA)的作用机制。方法造成免疫介导 AA 小鼠模型,第15天从正常组、模型组及叶绿素铜钠盐组小鼠脾脏分别提取 mRNA,经反转录分别用 Cy_3、Cy_5荧光标记,获得 cDNA 探针,与博星基因芯片表达谱杂交,扫描仪扫描结果,用分析软件分析统计。结果模型组和正常组差异表达基因共100条(4.88%),其中 AA 时上调基因29条,下调基因71条。模型组和叶绿素铜钠盐组差异表达基因共53条(2.59%),其中上调基因25条,下调基因28条。结论叶绿素铜钠盐通过下调模型小鼠脾脏 H2-Bf 的 mRNA 基因表达,上调 CASH 基因表达达到治疗 AA 的目的。

Experimental study of Chlorophyllin Capper Sodium Table on Gene Expression Profile of Immune Mediated Aplastic Anemia

Objective To explore the effective mechanism of Chlorophyllin Capper Sodium Table(CCS)for AA.Methods Immune-in- duced AA mice model was established.Thirty Balb/c mice were randomly divided into 3 groups,therapeutic group was gasterogavaged by CCS, AA model group and normal group by salinn.Spleens were obtained from 3 groups on fifteenth day,the fluorescent labeled cDNA probes were pre- pared from mRNA which were extracted from spleens of normal group and AA group and CCS group.Two kinds of probes were then hybridized a- gainst Biostar Gene chip including 2048 genes,The fluorescent signals were scanned and analyzed.Results There were 100 differentially ex- pressed genes(4.88%)between normal group and AA group.The expression level of 71 genes was down-regulated while 29 genes up-regu- lated significantly in spleens in mice of immune-induced AA.There were 53 differentially expressed genes(2.59%)between CCS group and AA group.The expression level of 28 genes was down-regulated while 25 genes up-regulated significantly in spleens in mice of immune-in- duced AA.Conclusion CCS treats AA through decreasing the expression level of H2-Bf and increasing the expression level of CASH.