小鼠骨髓瘤细胞系(SP_(2/0)—Ag_(14))及鼠一鼠淋巴细胞杂交瘤细胞系的超微形态观察

不分泌型的小鼠骨髓瘤细胞与其产生特异性抗体的杂交瘤细胞在光镜下难以区别其形态特征。但在电镜下,两种细胞系的细胞大小。胞浆内分泌细胞器的形态及数量均有明显差别:杂交瘤细胞的胞体明显增大,胞浆更为丰富,粗面内质网池扩张明显,高尔基区分泌小泡的数量明显增加,线粒体增大增多,暗细胞数量增加等。这些变化暗示,杂交瘤细胞系的分泌细胞器更为发达,分泌力增强。不分泌型的亲本细胞已转变成了分泌旺盛的细胞。但仅从形态上鉴别分泌物的性质较为困难。必须进一步结合免疫电镜技术验证。

OBSERVATION OF ULTRAMORPHOLOGY ON THE MOUSE MYELOMA CELL LINE(SP_(2/0)-Ag_(14))AND MOUSE-MOUSE LYMPHOCYTIC HYBRIDOMAS CELL LINE

Under light microscope,it is difficult to distingush non-secretory mouse myeloma cell line(SP2/0-Ag14)from the cells of its hybridoma secreting specific antibody. However under electron microscope,these two kinds of cells are distinctly different not only in the size but also in the amount and forms of the secretory organelles. In hybridoma,as compared with its parental,the cells significantly enlarged,the cytoplasm was more aboundent,the cisternae of rough surface endoplastic reticulum (RER)were apparently dilated,the number of the secretory vesicles of the Golgi apparatus was notably increased,the mitochondria became bigger and more aboundant, and the dark cells were increased.These changes suggest that the secretory organelles in hybridoma cells are more evalutive than those in parental cells,and the non-secretory parental cells have been transformed into actively secretory cells.But morphologically,it is difficult to identify the nature of the secretory products. Immunoelectron microscopy might be required for further confirmation.