日本血吸虫感染致肝纤维化家兔肝脏微循环病变观察

目的 研究日本血吸虫感染致肝纤维化家兔的肝脏微循环病变。方法 对30只日本血吸虫感染致肝纤维化家兔和10只正常家兔取肝组织行光镜和电镜观察肝脏微循环病变。结果 HE染色示汇管区可见虫卵沉积，虫卵周围炎性细胞浸润。Mallory三色染色见肝内门静脉、肝动脉管壁纤维结缔组织增多，中膜平滑肌增殖肥大。透射电镜下见肝窦内皮受损严重，细胞大多崩解、破裂，窦内可见炎性细胞浸润，毛细胆管扩张，肝细胞肝窦面的微绒毛减少或断裂，肝窦呈毛细血管化。结论 肝脏微循环障碍是形成血吸虫肝纤维化门脉高压的发病基础之一。

Hepatic Microcirculatory Pathology of Hepatic Cirrhotic Rabbit Induced by Infection of Schistosoma Japonicum

Objective To investigate the hepatic microcirculatory pathology of hepatic cirrhotic rabbits induced by infection of Schistosoma japonicum . Methods Liver tissues from 30 hepatic cirrhotic rabbits infected by Schistosoma japonicum and 10 normal rabbits were removed and observed under light and electron microscopy for hepatic microcirculatory pathology. Results Hematoxylin and Eosin (HE) staining indicated schistosome egg aggradation in the hilum and inflammatory cell infiltration around the schistosome eggs. Mallory trichrome staining showed hyperplasia of portal vein and hepatic artery wall fiber connective tissue, and proliferation and hypertrophy of the smooth muscle of middle membrane. Under transmission electron microscopy, it was found there was serious damage of hepatic sinusoidal endothelium cells, collapse of most cells, infiltration of inflammatory cells in hepatic sinusoidal, expansion of small bile duct, decreased or injured microfloss, and capillarization of hepatic sinusoidal.Conclusion Hepatic microcirculatory disturbances is one of the mechanisms by which schistosomiasis liver fibrosis induced portal hypertension develops.