肺腺癌血管内皮生长因子D表达与微血管密度、微淋巴管密度及淋巴转移的关系

目的 探讨肺腺癌中血管内皮生长因子D(VEGF-D)与微血管密度(MVD)、微淋巴管密度(MLVD)及淋巴结转移之间的关系.方法 RT-PCR检测VEGF-D mRNA在48例肺腺癌新鲜组织中的表达.免疫组化检测48例肺腺痛组织中VEGF-D蛋白的表达、MVD、MLVD.结果 VEGF-DmRNA在肺腺癌组织中高表达,在正常肺组织低表达(P<0.01).肿瘤周边部位VEGF-D表达显著高于肿瘤中心部位(P=0.014),其表达与肿瘤分化程度无关(P=0.624),与肿瘤的TNM分期有关,Ⅲ～Ⅳ期显著高于Ⅰ～Ⅱ期(P=0.015).MVD在VEGF-D阳性组和阴性组差异无统计学意义(P=0.408),VEGF-D阳性组MLVD显著高于阴性组(P<0.01),淋巴结转移增多(P=0.012).结论 VEGF-D的表达与肺腺痛淋巴管生成和淋巴结转移相关,而与血管生成无关.

Relation of vascular endothelial growth factor-D expression to microvessel density, microlymphatic vessel density, and lymph-node metastasis of lung adenocarcinoma

Objective To investigate the relationship of expression of vascular endothelial growth factor(VEGF)-D to microvessel density(MVD), microlymphatic vessel density (MLVD), and lymph node metastasis in lung adenocarcinoma. Methods Fresh specimens of lung adenocarcinoma were collected form 48 patients with lung adenocarcinoma, 28 males and 20 females, aged 35 -78, during operation. Semi- quantitative RT-PCR was used to detect the mRNA expression of VEGF-D, and immunohistochemistry was used to detect the protein expression of VEGF-D, CD34 (marker of MVD) , and VEGF receptor (VEGFR)- 3 (marker of MLV). Results The expression of VEGF-D mRNA in the lung cancer was significantly higher than that in the normal lung tissue, and the expression of VEGF-D protein in the cancerous invasive edge was significantly higher than that in the center of cancerous tissues. Twenty-one of the 29 lung adenocarcinomu patients at stages Ⅲ - Ⅳ were VEGF-D positive, a proportion significantly higher than that of the patients at stages Ⅰ - Ⅱ (7/19, P =0.015). The MLVD of the VEGF-D positive group was 10.25 ± 2. 50, significantly higher than that of the VEGF-D negative group (6. 8±2. 2, P <0. 01 ). Twenty-three of the 28 VEGF-D positive patients showed lymph node metastasis, a proportion significantly higher than that of the VEGF-D negative group (9/20, P = 0. 012). Conclusion VEGF-D is related with the lymphangiogenisis and lymph node metastasis in lung adenocarcinoma, but not with angiogenisis.