| 以甲壳胺为载体的固定化嗜热菌蛋白酶的研究 |
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| 引用本文： | 陶国良 陈震华. 以甲壳胺为载体的固定化嗜热菌蛋白酶的研究[J]. 医学教育探索, 1989, 0(2): 115-120 |
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| 作者姓名： | 陶国良 陈震华 |
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| 作者单位： | 武汉大学化学系 武汉(陶国良，陈震华)，武汉大学化学系 武汉(卓仁禧) |
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| 摘 要： | 本文报道了以甲壳胺为载体,采用戊二醛交联的方法将嗜热菌蛋白酶进行固定化。以酪蛋白为废物。固定化酶的活力回收为38％。本工作研究了各种因素对固定化酶活力的影响,实验结果表明:固定化后的酶,在热稳定性、抗抑制作用等方面较原酶有一定的提高。
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| 关 键 词： | 嗜热菌蛋白酶 甲壳胺 固定化 |
Study on Immobilized Thermolysin with Chitosan as Carrier |
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| Tao Guoliang,Chen Zhenhu,Zhuo Renxi Department of Chemistry,Wuhan University,Wuhan. Study on Immobilized Thermolysin with Chitosan as Carrier[J]. Researches in Medical Education, 1989, 0(2): 115-120 |
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| Authors: | Tao Guoliang Chen Zhenhu Zhuo Renxi Department of Chemistry Wuhan University Wuhan |
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| Affiliation: | Tao Guoliang,Chen Zhenhu,Zhuo Renxi Department of Chemistry,Wuhan University,Wuhan |
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| Abstract: | In this paper, immobilized thermolysin was prepared by using chitosan as carrier,and glutaraldehyde as crosslinking agent. The activity recover of the immobilizedthermolysin reached 38% when casein was used as the substrate. The properties ofthermolysin b |
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| Keywords: | thermolysin chitosan immobilization activity of enzyme |
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